| Genetic suppressors of Deltagrx3 Deltagrx4, lacking redundant multidomain monothiol yeast glutaredoxins, rescue growth and iron homeostasis. | Genetic suppressors of Δgrx3 Δgrx4, lacking redundant multidomain monothiol yeast glutaredoxins, rescue growth and iron homeostasis.
Li G, Nanjaraj Urs AN, Dancis A, Zhang Y., Free PMC Article | 06/18/2022 |
| Interactions of GMP with Human Glrx3 and with Saccharomyces cerevisiae Grx3 and Grx4 Converge in the Regulation of the Gcn2 Pathway. | Interactions of GMP with Human Glrx3 and with Saccharomyces cerevisiae Grx3 and Grx4 Converge in the Regulation of the Gcn2 Pathway.
Mechoud MA, Pujol-Carrion N, Montella-Manuel S, de la Torre-Ruiz MA., Free PMC Article | 11/21/2020 |
| The conserved CDC motif in the yeast iron regulator Aft2 mediates iron-sulfur cluster exchange and protein-protein interactions with Grx3 and Bol2 | The conserved CDC motif in the yeast iron regulator Aft2 mediates iron-sulfur cluster exchange and protein-protein interactions with Grx3 and Bol2.
Li H, Outten CE., Free PMC Article | 07/18/2020 |
| Study reports the crystal structure of both the Trx and Grx domains of Grx3, which enabled to model its full-length structure with an inter-domain disulfide bond. Despite the Trx domain alone losses the typical Trx activity, it significantly augments the glutathione S-transferases activity of the Grx domain, most likely via the interdomain disulfide bond. Also, Grx3 cooperates with Fra2 to regulate the activity of Atf2. | Structural and Biochemical Insights into the Multiple Functions of Yeast Grx3.
Chi CB, Tang Y, Zhang J, Dai YN, Abdalla M, Chen Y, Zhou CZ. | 05/11/2019 |
| We provide new mechanistic insights into Grx3/4 regulation of Sir2 by S-deglutathionylation to increase cell resistance to stress. This finding offers news perspectives on monothiol Grxs in redox signaling, describing Sir2 as a physiological substrate regulated by S-glutathionylation. | Reversible glutathionylation of Sir2 by monothiol glutaredoxins Grx3/4 regulates stress resistance.
Vall-Llaura N, Reverter-Branchat G, Vived C, Weertman N, Rodríguez-Colman MJ, Cabiscol E. | 01/13/2018 |
| Slt2 form iron/sulphur bridged clusters with Grx3 and Grx4. | Physical interaction between the MAPK Slt2 of the PKC1-MAPK pathway and Grx3/Grx4 glutaredoxins is required for the oxidative stress response in budding yeast.
Pujol-Carrion N, Torre-Ruiz MA. | 12/23/2017 |
| This article reports mechanistic studies that investigate the role of exogenous glutathione in defining cluster chirality, ligand exchange, and the cluster transfer chemistry of Saccharomyces cerevisiae Grx3. | Role of protein-glutathione contacts in defining glutaredoxin-3 [2Fe-2S] cluster chirality, ligand exchange and transfer chemistry.
Sen S, Cowan JA. | 12/16/2017 |
| RNR cofactor biogenesis requires the ISC machinery to mature the Grx3/4 and Dre2 Fe-S proteins, which then function in iron and electron delivery to RNR, respectively. | The diferric-tyrosyl radical cluster of ribonucleotide reductase and cytosolic iron-sulfur clusters have distinct and similar biogenesis requirements.
Li H, Stümpfig M, Zhang C, An X, Stubbe J, Lill R, Huang M., Free PMC Article | 07/15/2017 |
| diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins.( | Investigation of in vivo diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins.
Zhang Y, Liu L, Wu X, An X, Stubbe J, Huang M., Free PMC Article | 01/28/2012 |
| The authors present data showing that both Grx3 and Grx4 have roles in actin cytoskeleton remodeling and in cellular defenses against oxidative stress caused by reactive oxygen species (ROS) accumulation. | Glutaredoxins Grx4 and Grx3 of Saccharomyces cerevisiae play a role in actin dynamics through their Trx domains, which contributes to oxidative stress resistance.
Pujol-Carrion N, de la Torre-Ruiz MA., Free PMC Article | 02/26/2011 |
| The ability of the Fra2-Grx3/4 complex to assemble a [2Fe-2S] cluster may act as a signal to control the iron regulon in response to cellular iron status in yeast. | The yeast iron regulatory proteins Grx3/4 and Fra2 form heterodimeric complexes containing a [2Fe-2S] cluster with cysteinyl and histidyl ligation.
Li H, Mapolelo DT, Dingra NN, Naik SG, Lees NS, Hoffman BM, Riggs-Gelasco PJ, Huynh BH, Johnson MK, Outten CE., Free PMC Article | 01/21/2010 |
| crystal structure of the thioredoxin-like domain determined at 1.5 A resolution. loop containing signature motif WAxxC is partially disordered, indicating greater flexibility compared with classical dithiol thioredoxins with a WCGPC active-site motif. | Structure of the thioredoxin-like domain of yeast glutaredoxin 3.
Gibson LM, Dingra NN, Outten CE, Lebioda L., Free PMC Article | 01/21/2010 |
| Grx3 and Grx4 are novel components required for Aft1 iron regulation that most likely occurs in the nucleus | Role of glutaredoxin-3 and glutaredoxin-4 in the iron regulation of the Aft1 transcriptional activator in Saccharomyces cerevisiae.
Ojeda L, Keller G, Muhlenhoff U, Rutherford JC, Lill R, Winge DR. | 01/21/2010 |