| Uncovering targeting priority to yeast peroxisomes using an in-cell competition assay. | Uncovering targeting priority to yeast peroxisomes using an in-cell competition assay.
Rosenthal M, Metzl-Raz E, Bürgi J, Yifrach E, Drwesh L, Fadel A, Peleg Y, Rapaport D, Wilmanns M, Barkai N, Schuldiner M, Zalckvar E., Free PMC Article | 10/24/2020 |
| The Ile264 amino acid residue in Pex5, which is adjacent to the FxxxW diaromatic domain that was previously shown to be crucial for recognizing AOx and Cat2 as cargos, is also important for the efficient import of Fox2 and Cta1. | The budding yeast Pex5p receptor directs Fox2p and Cta1p into peroxisomes via its N-terminal region near the FxxxW domain.
Rymer Ł, Kempiński B, Chełstowska A, Skoneczny M. | 12/7/2019 |
| Pex5p recognition by the ATPases associated with various cellular activities complex (AAA) depends on the presence of the ubiquitin moiety and is mediated by Pex1p. | Receptor recognition by the peroxisomal AAA complex depends on the presence of the ubiquitin moiety and is mediated by Pex1p.
Schwerter D, Grimm I, Girzalsky W, Erdmann R., Free PMC Article | 03/9/2019 |
| Cysteine-specific ubiquitination protects the peroxisomal import receptor Pex5p against proteasomal degradation. | Cysteine-specific ubiquitination protects the peroxisomal import receptor Pex5p against proteasomal degradation.
Schwartzkopff B, Platta HW, Hasan S, Girzalsky W, Erdmann R., Free PMC Article | 12/17/2016 |
| Surface plasmon resonance analysis revealed a bivalent interaction mode for the phosphorylated PTS1 receptor Pex5 and Pcs60. | Structural insights into cargo recognition by the yeast PTS1 receptor.
Hagen S, Drepper F, Fischer S, Fodor K, Passon D, Platta HW, Zenn M, Schliebs W, Girzalsky W, Wilmanns M, Warscheid B, Erdmann R., Free PMC Article | 02/6/2016 |
| The cytosolic domain of Pex22p stimulates the Pex4p-dependent ubiquitination of the PTS1-receptor (Pex5). | The cytosolic domain of Pex22p stimulates the Pex4p-dependent ubiquitination of the PTS1-receptor.
El Magraoui F, Schrötter A, Brinkmeier R, Kunst L, Mastalski T, Müller T, Marcus K, Meyer HE, Girzalsky W, Erdmann R, Platta HW., Free PMC Article | 11/7/2015 |
| The membrane-associated import receptor Pex5p together with its docking partner Pex14p forms a gated ion-conducting channel which can be opened to a diameter of about 9 nm by the cytosolic receptor-cargo complex. | The peroxisomal importomer constitutes a large and highly dynamic pore.
Meinecke M, Cizmowski C, Schliebs W, Krüger V, Beck S, Wagner R, Erdmann R. | 04/19/2010 |
| Pex2 mediates the Ubc4-dependent polyubiquitination whereas Pex12 facilitates the Pex4-dependent monoubiquitination of Pex5. | Pex2 and pex12 function as protein-ubiquitin ligases in peroxisomal protein import.
Platta HW, El Magraoui F, Bäumer BE, Schlee D, Girzalsky W, Erdmann R., Free PMC Article | 01/21/2010 |
| The RING domain of Pex10p exhibits E(3) ligase activity in vitro, in combination with the human E(2) enzyme UbcH5a, a homologue of ScUbc4p, but not when ScPex4p is used as an E(2) enzyme in the reaction. | Pex10p functions as an E3 ligase for the Ubc4p-dependent ubiquitination of Pex5p.
Williams C, van den Berg M, Geers E, Distel B. | 01/21/2010 |
| E2D1/2/3 (UbcH5a/b/c) are the mammalian functional counterparts of yeast/plant Pex4p | Members of the E2D (UbcH5) family mediate the ubiquitination of the conserved cysteine of Pex5p, the peroxisomal import receptor.
Grou CP, Carvalho AF, Pinto MP, Wiese S, Piechura H, Meyer HE, Warscheid B, Sá-Miranda C, Azevedo JE. | 01/21/2010 |
| Pex4p-dependent ubiquitination of Pex5p occurs on a cysteine residue | A conserved cysteine is essential for Pex4p-dependent ubiquitination of the peroxisomal import receptor Pex5p.
Williams C, van den Berg M, Sprenger RR, Distel B. | 01/21/2010 |
| Study demonstrated that either poly- or monoubiquitination of Pex5 is required for the ATP-dependent release of the protein from the peroxisomal membrane to the cytosol as part of the receptor cycle. | Ubiquitination of the peroxisomal import receptor Pex5p is required for its recycling.
Platta HW, El Magraoui F, Schlee D, Grunau S, Girzalsky W, Erdmann R., Free PMC Article | 01/21/2010 |
| the C terminus of Pex14p contains distinct Pex5p and one Pex7p binding sites and the N terminus could be involved in a Pex5p-Pex14p association inside the peroxisomal membrane | Yeast Pex14p possesses two functionally distinct Pex5p and one Pex7p binding sites.
Niederhoff K, Meindl-Beinker NM, Kerssen D, Perband U, Schäfer A, Schliebs W, Kunau WH. | 01/21/2010 |
| identified ubiquitinated forms of Pex5p in deletion mutants of those PEX genes that have been implicated in recycling of Pex5p from the peroxisomal membrane into the cytosol | Ubiquitination of the peroxisomal targeting signal type 1 receptor, Pex5p, suggests the presence of a quality control mechanism during peroxisomal matrix protein import.
Kiel JA, Emmrich K, Meyer HE, Kunau WH. | 01/21/2010 |
| Results demonstrate that the AAA peroxins mediate the ATP-dependent dislocation of the peroxisomal targeting signal-1 (PTS1) receptor from the peroxisomal membrane to the cytosol. | Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol.
Platta HW, Grunau S, Rosenkranz K, Girzalsky W, Erdmann R. | 01/21/2010 |
| Data suggest that the N-terminal half of Pex5p facilitates the import of the major matrix protein Fox1p, and can be functionally replaced by Pex18p. | Functional similarity between the peroxisomal PTS2 receptor binding protein Pex18p and the N-terminal half of the PTS1 receptor Pex5p.
Schäfer A, Kerssen D, Veenhuis M, Kunau WH, Schliebs W., Free PMC Article | 01/21/2010 |
| Pex5p monoubiquitination in wild type cells is required for the recycling of Pex5p from the peroxisome, but Ubc4p-mediated polyubiquitination of Pex5p in mutants may function as a disposal mechanism for Pex5p when it gets stuck in the import pathway | The Saccharomyces cerevisiae peroxisomal import receptor Pex5p is monoubiquitinated in wild type cells.
Kragt A, Voorn-Brouwer T, van den Berg M, Distel B. | 01/21/2010 |
| a translocation-competent state of the PTS1 receptor (Pex5p) enters the membrane via protein-lipid interactions before it tightly associates with other peroxins | Membrane association of the cycling peroxisome import receptor Pex5p.
Kerssen D, Hambruch E, Klaas W, Platta HW, de Kruijff B, Erdmann R, Kunau WH, Schliebs W. | 01/21/2010 |