| We analyzed the dynamic localization and interaction of Chs3 and Chs4 during cell division, and found that both proteins transiently co-localize and physically interact only during bud formation and later in a period during septum formation and cytokinesis | Septin-associated protein kinase Gin4 affects localization and phosphorylation of Chs4, the regulatory subunit of the Baker's yeast chitin synthase III complex.
Gohlke S, Heine D, Schmitz HP, Merzendorfer H. | 03/23/2019 |
| that Chs7 engages in functionally distinct interactions with Chs3 to first promote its folding and endoplasmic reticulum exit, and subsequently to regulate its activity at the plasma membrane | The chaperone Chs7 forms a stable complex with Chs3 and promotes its activity at the cell surface.
Dharwada ST, Dalton LE, Bean BDM, Padmanabhan N, Choi C, Schluter C, Davey M, Conibear E. | 01/5/2019 |
| Hof1 binds to the Sel1-like repeats (SLRs) of Chs4 via its F-BAR domain and inhibits Chs3-mediated chitin synthesis during cytokinesis. In addition, Hof1 is required for rapid accumulation as well as efficient removal of Chs4 at the division site. | Hof1 and Chs4 Interact via F-BAR Domain and Sel1-like Repeats to Control Extracellular Matrix Deposition during Cytokinesis.
Oh Y, Schreiter JH, Okada H, Wloka C, Okada S, Yan D, Duan X, Bi E., Free PMC Article | 07/28/2018 |
| Synthesis of chitin by Chs3, the chitin synthase responsible for chitosan layer formation, is dependent on the cis-prenyltransferase activity of Srt1, indicating that polyprenols are necessary to coordinate assembly of the spore wall layers. | Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae.
Hoffmann R, Grabińska K, Guan Z, Sessa WC, Neiman AM., Free PMC Article | 07/21/2018 |
| the mitotic spindle breakage observed in endocytosis mutants can be rescued either by deleting or inhibiting the activities of, CHS2, CHS3 and FKS1, which are involved in septum formation. The findings from our study highlight the importance of timely endocytosis of cytokinetic enzymes at the division site in safeguarding mitotic spindle integrity during mitotic exit. | Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit.
Chin CF, Tan K, Onishi M, Chew Y, Augustine B, Lee WR, Yeong FM., Free PMC Article | 03/25/2017 |
| data show that the oligomerization of Chs3 through its N-terminus is essential for proper protein trafficking and chitin synthesis and is therefore monitored intracellularly. | Oligomerization of the chitin synthase Chs3 is monitored at the Golgi and affects its endocytic recycling.
Sacristan C, Manzano-Lopez J, Reyes A, Spang A, Muñiz M, Roncero C. | 05/24/2014 |
| The anchoring of Chs3 to septins through Chs4 prevents the arrival of this protein at endocytic sites. | Neck compartmentalization as the molecular basis for the different endocytic behaviour of Chs3 during budding or hyperpolarized growth in yeast cells.
Sacristan C, Reyes A, Roncero C. | 08/11/2012 |
| the essential role of Bni4 is to target Glc7 to the neck and activate it toward substrates necessary for CSIII recruitment and synthesis of chitin at the bud neck | Protein phosphatase type 1 directs chitin synthesis at the bud neck in Saccharomyces cerevisiae.
Larson JR, Bharucha JP, Ceaser S, Salamon J, Richardson CJ, Rivera SM, Tatchell K., Free PMC Article | 01/21/2010 |
| Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3. | Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3.
Schmidt M, Drgon T, Bowers B, Cabib E. | 01/21/2010 |
| Results suggest that Chs4p promotes Chs3p translocation into the plasma membrane in a stable and active form, and that proper chitin synthase III turnover is maintained through the endocytic internalization of Chs3p. | Chitin synthase III requires Chs4p-dependent translocation of Chs3p into the plasma membrane.
Reyes A, Sanz M, Duran A, Roncero C. | 01/21/2010 |
| We propose that Ypt31/32p have a role in regulating the delivery of Chs3p to the plasma membrane and deposition of chitin at the cell surface. | Ypt32p regulates the translocation of Chs3p from an internal pool to the plasma membrane.
Ortiz D, Novick PJ. | 01/21/2010 |