| Hog1 acts in a Mec1-independent manner to counteract oxidative stress following telomerase inactivation in Saccharomyces cerevisiae. | Hog1 acts in a Mec1-independent manner to counteract oxidative stress following telomerase inactivation in Saccharomyces cerevisiae.
Zeinoun B, Teixeira MT, Barascu A., Free PMC Article | 07/11/2024 |
| Hydroxyurea and inactivation of checkpoint kinase MEC1 inhibit transcription termination and pre-mRNA cleavage at polyadenylation sites in budding yeast. | Hydroxyurea and inactivation of checkpoint kinase MEC1 inhibit transcription termination and pre-mRNA cleavage at polyadenylation sites in budding yeast.
Kaur P, Nagar S, Mehta R, Sahadeo K, Vancura A., Free PMC Article | 08/19/2023 |
| Deletion of MEC1 suppresses the replicative senescence of the cdc13-2 mutant in Saccharomyces cerevisiae. | Deletion of MEC1 suppresses the replicative senescence of the cdc13-2 mutant in Saccharomyces cerevisiae.
Yao Y, Fekete-Szücs E, Rosas Bringas FR, Chang M., Free PMC Article | 05/12/2023 |
| Mec1 regulates PAS recruitment of Atg13 via direct binding with Atg13 during glucose starvation-induced autophagy. | Mec1 regulates PAS recruitment of Atg13 via direct binding with Atg13 during glucose starvation-induced autophagy.
Yao W, Li Y, Chen Y, Chen Y, Zhao P, Zhang Y, Jiang Q, Feng Y, Yang F, Wu C, Zhong H, Zhou Y, Sun Q, Zhang L, Liu W, Yi C., Free PMC Article | 12/31/2022 |
| Novel insights into the mechanism of cell cycle kinases Mec1(ATR) and Tel1(ATM). | Novel insights into the mechanism of cell cycle kinases Mec1(ATR) and Tel1(ATM).
Tannous EA, Burgers PM., Free PMC Article | 01/22/2022 |
| Budding yeast Rad51: a paradigm for how phosphorylation and intrinsic structural disorder regulate homologous recombination and protein homeostasis. | Budding yeast Rad51: a paradigm for how phosphorylation and intrinsic structural disorder regulate homologous recombination and protein homeostasis.
Woo TT, Chuang CN, Wang TF., Free PMC Article | 09/4/2021 |
| Mechanism of auto-inhibition and activation of Mec1(ATR) checkpoint kinase. | Mechanism of auto-inhibition and activation of Mec1(ATR) checkpoint kinase.
Tannous EA, Yates LA, Zhang X, Burgers PM., Free PMC Article | 03/13/2021 |
| Yeast ATM and ATR kinases use different mechanisms to spread histone H2A phosphorylation around a DNA double-strand break. | Yeast ATM and ATR kinases use different mechanisms to spread histone H2A phosphorylation around a DNA double-strand break.
Li K, Bronk G, Kondev J, Haber JE., Free PMC Article | 10/24/2020 |
| Mec1 Is Activated at the Onset of Normal S Phase by Low-dNTP Pools Impeding DNA Replication. | Mec1 Is Activated at the Onset of Normal S Phase by Low-dNTP Pools Impeding DNA Replication.
Forey R, Poveda A, Sharma S, Barthe A, Padioleau I, Renard C, Lambert R, Skrzypczak M, Ginalski K, Lengronne A, Chabes A, Pardo B, Pasero P. | 08/29/2020 |
| TORC1, Tel1/Mec1, and Mpk1 regulate autophagy induction after DNA damage in budding yeast. | TORC1, Tel1/Mec1, and Mpk1 regulate autophagy induction after DNA damage in budding yeast.
Ueda S, Ozaki R, Kaneko A, Akizuki R, Katsuta H, Miura A, Matsuura A, Ushimaru T. | 08/22/2020 |
| the C-terminal of Ddc1 has structural flexibility and it plays a critical role for Mec1/Ddc2 activation in G1/G2 phase; the 9-1-1 complex and Rad24-RFC interact | The structure of the checkpoint clamp 9-1-1 complex and clamp loader Rad24-RFC in Saccharomyces cerevisiae.
Liu W. | 07/11/2020 |
| The Dpb11 obtained by endogenous purification strongly stimulates the Mec1 kinase activity and promotes the changes in conformational distribution. This observation suggests the Dpb11 activates Mec1 kinase probably through modulation in the kinase conformations. | Preparation of endogenous TopBP1/Dpb11 and effect on central checkpoint kinase Mec1- Ddc2 (human ATR-ATRIP homolog).
Wu T, Zhang Z, Wang X. | 06/13/2020 |
| Mec1(ATR) was required for the formation of interhomolog joint molecules from early prophase, thus establishing a recombination partner choice. Moreover, Mec1(ATR) specifically promoted crossover-fated DNA double-strand breaks repair | Mec1 Modulates Interhomolog Crossover and Interplays with Tel1 at Post Double-Strand Break Stages.
Lee MS, Joo JW, Choi H, Kang HA, Kim K., Free PMC Article | 04/11/2020 |
| Study shows that Ddc2 promotes Mec1 activation independently of Ddc1/Dpb11/Dna2 function in vivo and through ssDNA recognition in vitro. The ddc2-S4 mutation diminishes damage-induced phosphorylation of the checkpoint mediators, Rad9 and Mrc1. Rad9 controls checkpoint throughout the cell-cycle whereas Mrc1 is specifically required for the S-phase checkpoint. | Ddc2ATRIP promotes Mec1ATR activation at RPA-ssDNA tracts.
Biswas H, Goto G, Wang W, Sung P, Sugimoto K., Free PMC Article | 01/11/2020 |
| that proteostasis is a fundamental function of Mec1 and that Mec1 is likely to utilize its checkpoint response network to mediate resistance to proteotoxic stress | Essential Function of Mec1, the Budding Yeast ATM/ATR Checkpoint-Response Kinase, in Protein Homeostasis.
Corcoles-Saez I, Dong K, Johnson AL, Waskiewicz E, Costanzo M, Boone C, Cha RS. | 09/1/2018 |
| Mec1 initiates checkpoint signaling, promotes DNA replication, and maintains genetic stability through distinct modes of action. | Separable roles for Mec1/ATR in genome maintenance, DNA replication, and checkpoint signaling.
Lanz MC, Oberly S, Sanford EJ, Sharma S, Chabes A, Smolka MB., Free PMC Article | 08/11/2018 |
| this study determined the structure of intact Mec1-Ddc2 (the yeast homolog of ATR-ATRIP), which is poised for catalysis, at a resolution of 3.9 angstroms. | 3.9 Å structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP.
Wang X, Ran T, Zhang X, Xin J, Zhang Z, Wu T, Wang W, Cai G. | 03/17/2018 |
| RPA-dependent recruitment of Mec1-Ddc2 is crucial for maintaining its homodimeric state at ssDNA and that Ddc2's recruitment domain and CCD are important for Mec1-dependent survival of UV-light-induced DNA damage. | Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage.
Deshpande I, Seeber A, Shimada K, Keusch JJ, Gut H, Gasser SM. | 10/28/2017 |
| Our findings provide a model in which two different Tel2 pathways promote protein stabilization of Mec1 and Tel1 in budding yeast. | Two separate pathways regulate protein stability of ATM/ATR-related protein kinases Mec1 and Tel1 in budding yeast.
Goto GH, Ogi H, Biswas H, Ghosh A, Tanaka S, Sugimoto K., Free PMC Article | 09/23/2017 |
| hese unusual mcm2DENQ phenotypes are markedly similar to those of a special previously-studied allele of the checkpoint sensor kinase ATR/MEC1, suggesting a possible regulatory interplay between Mcm2-7 and ATR during unchallenged growth. | A Checkpoint-Related Function of the MCM Replicative Helicase Is Required to Avert Accumulation of RNA:DNA Hybrids during S-phase and Ensuing DSBs during G2/M.
Vijayraghavan S, Tsai FL, Schwacha A., Free PMC Article | 05/27/2017 |
| Mec1 is highly activated during normal DNA replication. | Phosphoproteomics reveals distinct modes of Mec1/ATR signaling during DNA replication.
Bastos de Oliveira FM, Kim D, Cussiol JR, Das J, Jeong MC, Doerfler L, Schmidt KH, Yu H, Smolka MB., Free PMC Article | 04/30/2016 |
| Mms21 was phosophorylated during S-phase in a manner dependent on the DNA damage kinase Mec1. S260 S261 phosphorylation may positively regulate the SUMO ligase activity of Mms21 and promote genomic stability. | Mec1-dependent phosphorylation of Mms21 modulates its SUMO ligase activity.
Carlborg KK, Kanno T, Carter SD, Sjögren C. | 01/23/2016 |
| RNA-processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA | RNA-processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA.
Manfrini N, Trovesi C, Wery M, Martina M, Cesena D, Descrimes M, Morillon A, d'Adda di Fagagna F, Longhese MP., Free PMC Article | 10/24/2015 |
| the SWI/SNF chromatin remodeling complex is capable of regulating Mec1 kinase activity | Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex.
Kapoor P, Bao Y, Xiao J, Luo J, Shen J, Persinger J, Peng G, Ranish J, Bartholomew B, Shen X., Free PMC Article | 10/10/2015 |
| identified a Mec1/ATR- and Dbf4-dependent replication checkpoint in budding yeast that prevents the earliest stage of recombination | ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast.
Blitzblau HG, Hochwagen A., Free PMC Article | 07/25/2015 |