| An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-alpha to Mcm2-7. | An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-α to Mcm2-7.
Perez-Arnaiz P, Bruck I, Colbert MK, Kaplan DL., Free PMC Article | 10/21/2017 |
| The tethering of SCF(Dia2) to the replisome progression complex increases the efficiency of ubiquitylation of the Mcm7 subunit of CMG. Correspondingly, loss of tethering is synthetic lethal in combination with a disassembly-defective allele of CDC48. | Tethering of SCF(Dia2) to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase.
Maculins T, Nkosi PJ, Nishikawa H, Labib K., Free PMC Article | 06/11/2016 |
| The data demonstrate that ORC/Cdc6 facilitates directly MCM2-7 dimerization and shows that productive MCM2-7 dimerization is essential for prereplicative complex formation. | The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation.
Evrin C, Fernández-Cid A, Riera A, Zech J, Clarke P, Herrera MC, Tognetti S, Lurz R, Speck C., Free PMC Article | 05/10/2014 |
| origin ssDNA may stimulate the formation of the CMG complex by alleviating inhibitory interactions between Sld2 with Mcm2-7. | Enabling association of the GINS protein tetramer with the mini chromosome maintenance (Mcm)2-7 protein complex by phosphorylated Sld2 protein and single-stranded origin DNA.
Bruck I, Kanter DM, Kaplan DL., Free PMC Article | 12/10/2011 |
| origin single-stranded DNA releases Sld3 from Mcm2-7, allowing GINS to bind Mcm2-7. | Origin single-stranded DNA releases Sld3 protein from the Mcm2-7 complex, allowing the GINS tetramer to bind the Mcm2-7 complex.
Bruck I, Kaplan DL., Free PMC Article | 07/30/2011 |
| The minimal functional unit of Mcm4/6/7 is a hexamer | The effects of oligomerization on Saccharomyces cerevisiae Mcm4/6/7 function.
Ma X, Stead BE, Rezvanpour A, Davey MJ., Free PMC Article | 01/15/2011 |
| Analsis of the contribution of two structural motifs, the Walker B box and arginine finger, make to each Mcm2-7 ATPase active site. | The Saccharomyces cerevisiae Mcm6/2 and Mcm5/3 ATPase active sites contribute to the function of the putative Mcm2-7 'gate'.
Bochman ML, Schwacha A., Free PMC Article | 11/6/2010 |
| DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA. | Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.
Remus D, Beuron F, Tolun G, Griffith JD, Morris EP, Diffley JF., Free PMC Article | 01/21/2010 |
| Data indicate that a complex of the GINS and Ctf4 components of the replisome progression complex is crucial to couple MCM2-7 to DNA polymerase alpha. | A key role for Ctf4 in coupling the MCM2-7 helicase to DNA polymerase alpha within the eukaryotic replisome.
Gambus A, van Deursen F, Polychronopoulos D, Foltman M, Jones RC, Edmondson RD, Calzada A, Labib K., Free PMC Article | 01/21/2010 |
| incorporation of Mcm2-7 into the prereplicative complex increased the level and changes the specificity of Dbf4-dependent kinase phosphorylation of this complex | Incorporation into the prereplicative complex activates the Mcm2-7 helicase for Cdc7-Dbf4 phosphorylation.
Francis LI, Randell JC, Takara TJ, Uchima L, Bell SP., Free PMC Article | 01/21/2010 |
| The Mcm4/Mcm7 and Mcm4/Mcm6/Mcm7 assemblies can open to load onto circular DNA to initiate unwinding. | Mcm subunits can assemble into two different active unwinding complexes.
Kanter DM, Bruck I, Kaplan DL., Free PMC Article | 01/21/2010 |
| Cdc7-Dbf4 phosphorylation of the MCM complex normally results in a single, highly active conformation of Mcm5, whereas the mcm5-bob1 mutation produces a number of conformations, only one of which is permissive for origin activation. | Structural changes in Mcm5 protein bypass Cdc7-Dbf4 function and reduce replication origin efficiency in Saccharomyces cerevisiae.
Hoang ML, Leon RP, Pessoa-Brandao L, Hunt S, Raghuraman MK, Fangman WL, Brewer BJ, Sclafani RA., Free PMC Article | 01/21/2010 |