| Conformational dynamics of cohesin/Scc2 loading complex are regulated by Smc3 acetylation and ATP binding. | Conformational dynamics of cohesin/Scc2 loading complex are regulated by Smc3 acetylation and ATP binding.
Kaushik A, Than T, Petela NJ, Voulgaris M, Percival C, Daniels P, Rafferty JB, Nasmyth KA, Hu B., Free PMC Article | 09/28/2023 |
| A model for Scc2p stimulation of cohesin's ATPase and its inhibition by acetylation of Smc3p. | A model for Scc2p stimulation of cohesin's ATPase and its inhibition by acetylation of Smc3p.
Boardman K, Xiang S, Chatterjee F, Mbonu U, Guacci V, Koshland D., Free PMC Article | 04/26/2023 |
| DNA passes through cohesin's hinge as well as its Smc3-kleisin interface. | DNA passes through cohesin's hinge as well as its Smc3-kleisin interface.
Collier JE, Nasmyth KA., Free PMC Article | 10/1/2022 |
| Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops. | Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops.
Bastié N, Chapard C, Dauban L, Gadal O, Beckouët F, Koszul R. | 06/25/2022 |
| The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks. | The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks.
van Schie JJM, de Lange J., Free PMC Article | 01/22/2022 |
| Functional control of Eco1 through the MCM complex in sister chromatid cohesion. | Functional control of Eco1 through the MCM complex in sister chromatid cohesion.
Yoshimura A, Sutani T, Shirahige K. | 05/1/2021 |
| ATP-binding and Smc1-Smc3 heterodimerization promote conformational changes within the ATPase that are transmitted to the Smc coiled-coil domains. | The structure of the cohesin ATPase elucidates the mechanism of SMC-kleisin ring opening.
Muir KW, Li Y, Weis F, Panne D., Free PMC Article | 04/18/2020 |
| The authors show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1's ATPase. | Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1.
Srinivasan M, Petela NJ, Scheinost JC, Collier J, Voulgaris M, B Roig M, Beckouët F, Hu B, Nasmyth KA., Free PMC Article | 02/22/2020 |
| The X. laevis Eco2 bound to its primary and secondary Smc3 substrates demonstrate the plasticity of the substrate-binding site, which confers substrate specificity by concerted conformational changes of the central beta-hairpin and the C-terminal extension. | Structural Basis of Eco1-Mediated Cohesin Acetylation.
Chao WC, Wade BO, Bouchoux C, Jones AW, Purkiss AG, Federico S, O'Reilly N, Snijders AP, Uhlmann F, Singleton MR., Free PMC Article | 11/10/2018 |
| Smc3 deacetylation by Hos1, triggered by Scc1 cleavage, facilitates efficient dissolution of sister chromatid cohesion during early metaphase. | Smc3 Deacetylation by Hos1 Facilitates Efficient Dissolution of Sister Chromatid Cohesion during Early Anaphase.
Li S, Yue Z, Tanaka TU., Free PMC Article | 11/25/2017 |
| A reversible association between Smc1 and Smc3 coiled coils is regulated by lysine acetylation and is required for cohesin association with the DNA. | A Reversible Association between Smc Coiled Coils Is Regulated by Lysine Acetylation and Is Required for Cohesin Association with the DNA.
Kulemzina I, Ang K, Zhao X, Teh JT, Verma V, Suranthran S, Chavda AP, Huber RG, Eisenhaber B, Eisenhaber F, Yan J, Ivanov D. | 09/9/2017 |
| We identified a large number of mutations in the CC region of both Smc1 and Smc3... we introduced them to the yeast Smc1 and Smc3 CC domains and characterized the effect of these mutant alleles on cohesin's function. We identified a missense mutation in the region of the kink domain of Smc3, which was previously identified in kidney carcinoma | Identification of a region in the coiled-coil domain of Smc3 that is essential for cohesin activity.
Orgil O, Mor H, Matityahu A, Onn I., Free PMC Article | 06/24/2017 |
| These results also suggest that Eco1 promotes cohesion by modulating the ATPase cycle of DNA-bound cohesin in a state that is permissive for DNA tethering and refractory to Wpl1 inhibition. | The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering.
Çamdere G, Guacci V, Stricklin J, Koshland D., Free PMC Article | 10/8/2016 |
| Study shows that cohesin's release from the DNA is mediated by disengagement of the Smc3/Scc1 interface involving interaction between the SMC ATPase heads and is inhibited by Smc3 acetylation. | Releasing Activity Disengages Cohesin's Smc3/Scc1 Interface in a Process Blocked by Acetylation.
Beckouët F, Srinivasan M, Roig MB, Chan KL, Scheinost JC, Batty P, Hu B, Petela N, Gligoris T, Smith AC, Strmecki L, Rowland BD, Nasmyth K., Free PMC Article | 07/30/2016 |
| Smc5/6 and cohesin complexes are interrelated and cooperating factors that control chromosome dynamics throughout interphase. [review] | Functional interplay between cohesin and Smc5/6 complexes.
Tapia-Alveal C, Lin SJ, O'Connell MJ., Free PMC Article | 05/23/2015 |
| Data indicate that cell cycle protein Pds5 promotes acetylation of cohesin subunit Smc3 by acetyltransferase Eco1 during S phase. | Pds5 promotes and protects cohesin acetylation.
Chan KL, Gligoris T, Upcher W, Kato Y, Shirahige K, Nasmyth K, Beckouët F., Free PMC Article | 11/16/2013 |
| Sister chromatid cohesion is mediated by entrapment of sister DNAs by a tripartite ring composed of cohesin's Smc1, Smc3, and alpha-kleisin subunits; the function of Smc3 acetylation is to block its dissociation from alpha-kleisin | Cohesin's DNA exit gate is distinct from its entrance gate and is regulated by acetylation.
Chan KL, Roig MB, Hu B, Beckouët F, Metson J, Nasmyth K., Free PMC Article | 11/17/2012 |
| by retarding Topo II-driven decatenation, cohesin (Smc1, Smc3, Scc1) mediates sister chromatid cohesion by an indirect mechanism as well as one involving entrapment of sister DNAs inside its tripartite ring | Cohesin's concatenation of sister DNAs maintains their intertwining.
Farcas AM, Uluocak P, Helmhart W, Nasmyth K., Free PMC Article | 12/24/2011 |
| Data provide evidence that Smc3p acetylation, which has an essential role in cohesion establishment, modulates the Smc3p ATP-bound state. | Genetic evidence that the acetylation of the Smc3p subunit of cohesin modulates its ATP-bound state to promote cohesion establishment in Saccharomyces cerevisiae.
Heidinger-Pauli JM, Onn I, Koshland D., Free PMC Article | 02/26/2011 |
| A cycle of Smc3 NBD acetylation is therefore an essential aspect of the chromosome cycle in eukaryotic cells | An Smc3 acetylation cycle is essential for establishment of sister chromatid cohesion.
Beckouët F, Hu B, Roig MB, Sutani T, Komata M, Uluocak P, Katis VL, Shirahige K, Nasmyth K., Free PMC Article | 10/23/2010 |
| Hos1 deacetylates Smc3 to close the cohesin acetylation cycle. | Hos1 deacetylates Smc3 to close the cohesin acetylation cycle.
Borges V, Lehane C, Lopez-Serra L, Flynn H, Skehel M, Rolef Ben-Shahar T, Uhlmann F. | 10/23/2010 |
| study found that the head domain of the Smc3p subunit of cohesin is acetylated by the Eco1p acetyltransferase at two evolutionarily conserved residues, promoting the chromatin-bound cohesin to tether sister chromatids | A molecular determinant for the establishment of sister chromatid cohesion.
Unal E, Heidinger-Pauli JM, Kim W, Guacci V, Onn I, Gygi SP, Koshland DE. | 01/21/2010 |
| findings show that Smc3 is acetylated in an Eco1-dependent manner during DNA replication to promote sister chromatid cohesion | Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion.
Rolef Ben-Shahar T, Heeger S, Lehane C, East P, Flynn H, Skehel M, Uhlmann F. | 01/21/2010 |
| This study identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion. | Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast.
Zhang J, Shi X, Li Y, Kim BJ, Jia J, Huang Z, Yang T, Fu X, Jung SY, Wang Y, Zhang P, Kim ST, Pan X, Qin J. | 01/21/2010 |
| We provide molecular evidence that cohesins are required for repair of replication-born DSBs by SCE, as determined in smc3, scc1 and scc2 mutants, but not for other recombinational repair events. | Double-strand breaks arising by replication through a nick are repaired by cohesin-dependent sister-chromatid exchange.
Cortés-Ledesma F, Aguilera A., Free PMC Article | 01/21/2010 |