| Flap endonuclease Rad27 cleaves the RNA of R-loop structures to suppress telomere recombination. | Flap endonuclease Rad27 cleaves the RNA of R-loop structures to suppress telomere recombination.
Liu CC, Chan HR, Su GC, Hsieh YZ, Lei KH, Kato T, Yu TY, Kao YW, Cheng TH, Chi P, Lin JJ., Free PMC Article | 06/1/2023 |
| Rad27 and Exo1 function in different excision pathways for mismatch repair in Saccharomyces cerevisiae. | Rad27 and Exo1 function in different excision pathways for mismatch repair in Saccharomyces cerevisiae.
Calil FA, Li BZ, Torres KA, Nguyen K, Bowen N, Putnam CD, Kolodner RD., Free PMC Article | 10/23/2021 |
| Cross-Species Complementation of Nonessential Yeast Genes Establishes Platforms for Testing Inhibitors of Human Proteins. | Cross-Species Complementation of Nonessential Yeast Genes Establishes Platforms for Testing Inhibitors of Human Proteins.
Hamza A, Driessen MRM, Tammpere E, O'Neil NJ, Hieter P., Free PMC Article | 01/23/2021 |
| By conditionally depleting lagging-strand nucleases and directly analyzing Okazaki fragments synthesized in vivo in Saccharomyces cerevisiae, we conduct a systematic evaluation of the impact of Rad27, Dna2 and Exo1 on lagging-strand synthesis. We find that Rad27 processes the majority of lagging-strand flaps, with a significant additional contribution from Exo1 but not from Dna2. | Processing of eukaryotic Okazaki fragments by redundant nucleases can be uncoupled from ongoing DNA replication in vivo.
Kahli M, Osmundson JS, Yeung R, Smith DJ., Free PMC Article | 08/31/2019 |
| Overexpression of RAD27 impairs DNA replication leading to S phase delay, DNA damage and mutation in a manner that is dependent on its interaction with PCNA. | Flap endonuclease overexpression drives genome instability and DNA damage hypersensitivity in a PCNA-dependent manner.
Becker JR, Gallo D, Leung W, Croissant T, Thu YM, Nguyen HD, Starr TK, Brown GW, Bielinsky AK., Free PMC Article | 07/27/2019 |
| Data show that unlike short-flap substrates, which are bound, bent and cleaved within the first encounter between Flap Endonuclease 1 (FEN1) and DNA, long-flap substrates can escape cleavage even after DNA binding and bending. | Missed cleavage opportunities by FEN1 lead to Okazaki fragment maturation via the long-flap pathway.
Zaher MS, Rashid F, Song B, Joudeh LI, Sobhy MA, Tehseen M, Hingorani MM, Hamdan SM., Free PMC Article | 07/27/2019 |
| Rad2 and rad27 overexpression increases genomic instability in yeast. Rad2 and Rad27 causes DNA breaks that are processed by different repair functions. | Differential effect of the overexpression of Rad2/XPG family endonucleases on genome integrity in yeast and human cells.
Jimeno S, Herrera-Moyano E, Ortega P, Aguilera A. | 12/30/2017 |
| Data show that wild-type Rad27 proteins are more stably associated with chromatin than tailless Rad27. | Physical and functional interactions between nucleosomes and Rad27, a critical component of DNA processing during DNA metabolism.
Kwon B, Munashingha PR, Shin YK, Lee CH, Li B, Seo YS. | 07/15/2017 |
| our findings underscore the importance of Rad27p in the maintenance of mtDNA, and demonstrate that it participates in multiple DNA repair pathways in mitochondria, unlinked to nuclear phenotypes. | Roles for the Rad27 Flap Endonuclease in Mitochondrial Mutagenesis and Double-Strand Break Repair in Saccharomyces cerevisiae.
Nagarajan P, Prevost CT, Stein A, Kasimer R, Kalifa L, Sia EA., Free PMC Article | 07/15/2017 |
| findings indicate that the N-terminal region of Mus81 acts as a landing pad to interact with Rad27 and that Mus81 and Rad27 work conjointly for efficient removal of various aberrant DNA structures | A physiological significance of the functional interaction between Mus81 and Rad27 in homologous recombination repair.
Thu HP, Nguyen TA, Munashingha PR, Kwon B, Dao Van Q, Seo YS., Free PMC Article | 06/20/2015 |
| C-terminal 16-amino acid fragment of Rad27 was sufficient and necessary for the stimulation of both Rad27 and Dna2. | The trans-autostimulatory activity of Rad27 suppresses dna2 defects in Okazaki fragment processing.
Munashingha PR, Lee CH, Kang YH, Shin YK, Nguyen TA, Seo YS., Free PMC Article | 06/16/2012 |
| High-copy expression of POL30 (PCNA) suppresses the canavanine mutation rate of all the rad27 alleles, including rad27Delta. | C-terminal flap endonuclease (rad27) mutations: lethal interactions with a DNA ligase I mutation (cdc9-p) and suppression by proliferating cell nuclear antigen (POL30) in Saccharomyces cerevisiae.
Karanja KK, Livingston DM., Free PMC Article | 02/1/2010 |
| Rad27p/FEN1 is localized in the mitochondrial compartment of both yeast and mice and that Rad27p has a significant role in maintaining mtDNA integrity. | Evidence for a role of FEN1 in maintaining mitochondrial DNA integrity.
Kalifa L, Beutner G, Phadnis N, Sheu SS, Sia EA., Free PMC Article | 01/25/2010 |
| the activity of Rad27p was critical for achieving full chronological lifespan | Mutations in the RAD27 and SGS1 genes differentially affect the chronological and replicative lifespan of yeast cells growing on glucose and glycerol.
Ringvoll J, Uldal L, Roed MA, Reite K, Baynton K, Klungland A, Eide L. | 01/21/2010 |
| Dna2 enables FEN1 to cleave RPA-coated flaps. In summary, RPA, Dna2, and FEN1 have evolved highly coordinated binding properties enabling one protein to succeed the next for proper and efficient Okazaki flap processing. | Significance of the dissociation of Dna2 by flap endonuclease 1 to Okazaki fragment processing in Saccharomyces cerevisiae.
Stewart JA, Campbell JL, Bambara RA., Free PMC Article | 01/21/2010 |
| lack of coordination between DNA polymerase delta and Rad27/Fen1 with the PCNA sliding clamp during long-patch base excision repair of alkylation damage can result in many double-strand breaks within the chromosomes of nondividing haploid cells | The transition of closely opposed lesions to double-strand breaks during long-patch base excision repair is prevented by the coordinated action of DNA polymerase delta and Rad27/Fen1.
Ma W, Panduri V, Sterling JF, Van Houten B, Gordenin DA, Resnick MA., Free PMC Article | 01/21/2010 |
| Biochemical fractionation studies identified FEN1 (Rad27) as another required DNA large loop repair component. | Partial reconstitution of DNA large loop repair with purified proteins from Saccharomyces cerevisiae.
Sommer D, Stith CM, Burgers PM, Lahue RS., Free PMC Article | 01/21/2010 |
| Combining rad27-p with cdc9-p increases the incidence of repeat tract expansion frequency above the sum of the numbers recorded in the individual mutants | Interactions among DNA ligase I, the flap endonuclease and proliferating cell nuclear antigen in the expansion and contraction of CAG repeat tracts in yeast.
Refsland EW, Livingston DM., Free PMC Article | 01/21/2010 |
| Pathological interactions between the rad27Delta and specific nupDelta mutations result from the accumulation of unrepaired DNA damages. | Genetic network interactions among replication, repair and nuclear pore deficiencies in yeast.
Loeillet S, Palancade B, Cartron M, Thierry A, Richard GF, Dujon B, Doye V, Nicolas A. | 01/21/2010 |