| A New Twist in ABC Transporter Mediated Multidrug Resistance - Pdr5 is a Drug/proton Co-transporter. | A New Twist in ABC Transporter Mediated Multidrug Resistance - Pdr5 is a Drug/proton Co-transporter.
Wagner M, Blum D, Raschka SL, Nentwig LM, Gertzen CGW, Chen M, Gatsogiannis C, Harris A, Smits SHJ, Wagner R, Schmitt L. | 07/16/2022 |
| Yeast Double Transporter Gene Deletion Library for Identification of Xenobiotic Carriers in Low or High Throughput. | Yeast Double Transporter Gene Deletion Library for Identification of Xenobiotic Carriers in Low or High Throughput.
Almeida LD, Silva ASF, Mota DC, Vasconcelos AA, Camargo AP, Pires GS, Furlan M, Freire HMRDC, Klippel AH, Silva SF, Zanelli CF, Carazzolle MF, Oliver SG, Bilsland E., Free PMC Article | 02/26/2022 |
| RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in rho(0) cells of Saccharomyces cerevisiae. | RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ(0) cells of Saccharomyces cerevisiae.
Yamada Y., Free PMC Article | 01/22/2022 |
| Structure and efflux mechanism of the yeast pleiotropic drug resistance transporter Pdr5. | Structure and efflux mechanism of the yeast pleiotropic drug resistance transporter Pdr5.
Harris A, Wagner M, Du D, Raschka S, Nentwig LM, Gohlke H, Smits SHJ, Luisi BF, Schmitt L., Free PMC Article | 09/25/2021 |
| In vitro NTPase activity of highly purified Pdr5, a major yeast ABC multidrug transporter. | In vitro NTPase activity of highly purified Pdr5, a major yeast ABC multidrug transporter.
Wagner M, Smits SHJ, Schmitt L., Free PMC Article | 10/31/2020 |
| Pdr5 has a newly discovered RNA stability element within its coding region. | Nonsynonymous Mutations in Linker-2 of the Pdr5 Multidrug Transporter Identify a New RNA Stability Element.
Rahman H, Rudrow A, Carneglia J, Joly SSP, Nicotera D, Naldrett M, Choy J, Ambudkar SV, Golin J., Free PMC Article | 06/13/2020 |
| Sequence comparison between the target proteins, i.e., the plant EPSPS and the yeast orthologous protein Aro1, predicted that yeast would be resistant to glyphosate. However, the growth variation seen in the subset of yeast tested was not due to polymorphisms within Aro1, instead, it was caused by genetic variation in an ABC multiple drug transporter, Pdr5, and an amino acid permease, Dip5 | Genetic variation in Dip5, an amino acid permease, and Pdr5, a multiple drug transporter, regulates glyphosate resistance in S. cerevisiae.
Rong-Mullins X, Ravishankar A, McNeal KA, Lonergan ZR, Biega AC, Creamer JP, Gallagher JEG., Free PMC Article | 12/9/2017 |
| These results present new evidence, suggesting that transmembrane helix 2 plays an important role for the efflux function of Pdr5p. | Mutations of charged amino acids at the cytoplasmic end of transmembrane helix 2 affect transport activity of the budding yeast multidrug resistance protein Pdr5p.
Dou W, Zhu J, Wang T, Wang W, Li H, Chen X, Guan W., Free PMC Article | 08/5/2017 |
| structural and functional implications for Pdr5 | Transmitting the energy: interdomain cross-talk in Pdr5.
Wagner M, Doehl K, Schmitt L. | 07/15/2017 |
| High-Copy Overexpression Screening Reveals PDR5 as the Main Doxorubicin Resistance Gene in Yeast. | High-Copy Overexpression Screening Reveals PDR5 as the Main Doxorubicin Resistance Gene in Yeast.
Demir AB, Koc A., Free PMC Article | 06/28/2016 |
| Multidrug resistance transporters Snq2p and Pdr5p mediate caffeine efflux in Saccharomyces cerevisiae.Caffeine was exported by Snq2p and Pdr5p. | Multidrug resistance transporters Snq2p and Pdr5p mediate caffeine efflux in Saccharomyces cerevisiae.
Tsujimoto Y, Shimizu Y, Otake K, Nakamura T, Okada R, Miyazaki T, Watanabe K. | 05/14/2016 |
| Our results demonstrated that organic solvent stress was likely to damage mitochondria, causing generation of reactive oxygen species and mitochondrial fragmentation, and to activate retrograde signaling pathway via Pdr3p to upregulate PDR5 expression. | Activation of the mitochondrial signaling pathway in response to organic solvent stress in yeast.
Nishida-Aoki N, Mori H, Kuroda K, Ueda M. | 01/2/2016 |
| Pdr5 analyses are applicable to mammalian asymmetric ABC multidrug transporters, including transporter promiscuity, function of non-catalytic ATP-binding site, description of a transmission interface, and discovery that Pdr5 is a molecular diode. Review. | The multidrug transporter Pdr5 on the 25th anniversary of its discovery: an important model for the study of asymmetric ABC transporters.
Golin J, Ambudkar SV., Free PMC Article | 06/27/2015 |
| Novel phenotype of an S1368A mutant that lies in the putative drug-binding pocket of the yeast multidrug transporter Pdr5. | Evidence for a molecular diode-based mechanism in a multispecific ATP-binding cassette (ABC) exporter: SER-1368 as a gatekeeping residue in the yeast multidrug transporter Pdr5.
Mehla J, Ernst R, Moore R, Wakschlag A, Marquis MK, Ambudkar SV, Golin J., Free PMC Article | 02/7/2015 |
| We describe here the first attempts to restore complete symmetry in an asymmetric ABC transporter and to study its effects, which might be relevant to the entire class of asymmetric ABC transporters. | Generating symmetry in the asymmetric ATP-binding cassette (ABC) transporter Pdr5 from Saccharomyces cerevisiae.
Gupta RP, Kueppers P, Hanekop N, Schmitt L., Free PMC Article | 10/11/2014 |
| Mutations adjacent to the end of transmembrane helices 6 and 7 independently affect drug efflux capacity of Pdr5p. | Mutations adjacent to the end of transmembrane helices 6 and 7 independently affect drug efflux capacity of yeast ABC transporter Pdr5p.
Chen Z, Li J, Wang W, Guo X, Li Y, Mao X, Chen X, Guan W. | 03/29/2014 |
| The deviant ATP-binding site of the multidrug efflux pump Pdr5 plays an active role in the transport cycle. | The deviant ATP-binding site of the multidrug efflux pump Pdr5 plays an active role in the transport cycle.
Furman C, Mehla J, Ananthaswamy N, Arya N, Kulesh B, Kovach I, Ambudkar SV, Golin J., Free PMC Article | 01/4/2014 |
| These results indicate that the S1360F mutation within the transmembrane domain interferes drastically with the ability of the nucleotide-binding domains to hydrolyze ATP by interfering with interdomain crosstalk. | Functional impact of a single mutation within the transmembrane domain of the multidrug ABC transporter Pdr5.
Kueppers P, Gupta RP, Stindt J, Smits SH, Schmitt L. | 05/25/2013 |
| A mutation in intracellular loop 4 affects the drug-efflux activity of the yeast multidrug resistance ABC transporter Pdr5p. | A mutation in intracellular loop 4 affects the drug-efflux activity of the yeast multidrug resistance ABC transporter Pdr5p.
Guo X, Li J, Wang T, Liu Z, Chen X, Li Y, Gu Z, Mao X, Guan W, Li Y., Free PMC Article | 05/19/2012 |
| The multidrug resistance transporters Pdr5p and Snq2p expel external compounds solely within the surface cell layers of biofilms. | Flo11p, drug efflux pumps, and the extracellular matrix cooperate to form biofilm yeast colonies.
Váchová L, Stovícek V, Hlavácek O, Chernyavskiy O, Stĕpánek L, Kubínová L, Palková Z., Free PMC Article | 01/28/2012 |
| Data show that tryptophan transport is increased in pdr5Delta/yor1 strains and reduced in rsb1Delta cells, and the localization and vacuolar degradation of Tat2 were affected in these genetic backgrounds. | Regulation of yeast nutrient permease endocytosis by ATP-binding cassette transporters and a seven-transmembrane protein, RSB1.
Johnson SS, Hanson PK, Manoharlal R, Brice SE, Cowart LA, Moye-Rowley WS., Free PMC Article | 02/26/2011 |
| Pdr5p and Snq2p transporters play important physiological roles and significantly influence the developmental phases and physiology of yeast populations growing in a liquid culture. | Putative role for ABC multidrug exporters in yeast quorum sensing.
Hlavácek O, Kucerová H, Harant K, Palková Z, Váchová L. | 01/21/2010 |
| drug sensitivity of mutant Pdr5 is attributable to the uncoupling of NTPase activity and transport | Mutations define cross-talk between the N-terminal nucleotide-binding domain and transmembrane helix-2 of the yeast multidrug transporter Pdr5: possible conservation of a signaling interface for coupling ATP hydrolysis to drug transport.
Sauna ZE, Bohn SS, Rutledge R, Dougherty MP, Cronin S, May L, Xia D, Ambudkar SV, Golin J., Free PMC Article | 01/21/2010 |
| Overproduction of Psd1 in wild-type ([rho(+)]) cells was found to induce PDR5 transcription and drug resistance in a Pdr3-dependent manner. | Evidence for the bifunctional nature of mitochondrial phosphatidylserine decarboxylase: role in Pdr3-dependent retrograde regulation of PDR5 expression.
Gulshan K, Schmidt JA, Shahi P, Moye-Rowley WS., Free PMC Article | 01/21/2010 |
| Vps3 has an epistatic relationship with Pdr5, a major yeast multidrug transporter. | Pdr5-mediated multidrug resistance requires the CPY-vacuolar sorting protein Vps3: are xenobiotic compounds routed from the vacuole to plasma membrane transporters for efflux?
Rutledge RM, Ghislain M, Mullins JM, de Thozée CP, Golin J. | 01/21/2010 |