| Dynamic 1D search and processive nucleosome translocations by RSC and ISW2 chromatin remodelers. | Dynamic 1D search and processive nucleosome translocations by RSC and ISW2 chromatin remodelers.
Kim JM, Carcamo CC, Jazani S, Xie Z, Feng XA, Yamadi M, Poyton M, Holland KL, Grimm JB, Lavis LD, Ha T, Wu C., Free PMC Article | 03/22/2024 |
| Basis of specificity for a conserved and promiscuous chromatin remodeling protein. | Basis of specificity for a conserved and promiscuous chromatin remodeling protein.
Donovan DA, Crandall JG, Truong VN, Vaaler AL, Bailey TB, Dinwiddie D, Banks OG, McKnight LE, McKnight JN., Free PMC Article | 01/29/2022 |
| Histone Octamer Structure Is Altered Early in ISW2 ATP-Dependent Nucleosome Remodeling. | Histone Octamer Structure Is Altered Early in ISW2 ATP-Dependent Nucleosome Remodeling.
Hada A, Hota SK, Luo J, Lin YC, Kale S, Shaytan AK, Bhardwaj SK, Persinger J, Ranish J, Panchenko AR, Bartholomew B. | 08/29/2020 |
| we report that Isw2 and Ino80 promote efficient firing of the ribosomal origin of replication and facilitate the regulated increase of rDNA repeat copy number. This work significantly expands our understanding of the importance of ATP-dependent chromatin remodeling for rDNA biology. | Chromatin Remodeling Factors Isw2 and Ino80 Regulate Chromatin, Replication, and Copy Number of the Saccharomyces cerevisiae Ribosomal DNA Locus.
Cutler S, Lee LJ, Tsukiyama T., Free PMC Article | 03/2/2019 |
| single mutations in genes ISW1, ISW2, and CHD1 weakly affect repair and mutagenic processes in yeast cells. | [The role of remodeling complexes CHD1 and ISWI in spontaneous and UV-induced mutagenesis control in yeast Saccharomyces cerevisiae].
Evstiukhina TA, Alekseeva EA, Fedorov DV, Peshekhonov VT, Korolev VG. | 02/17/2018 |
| Histone hyperacetylation does not necessarily block ISWI functions and that ISWI enzymes can function on active chromatin as well. | Mobilization of hyperacetylated mononucleosomes by purified yeast ISW2 in vitro.
Krajewski WA. | 06/28/2016 |
| the ISWI class of ATP-dependent chromatin remodeling complexes plays a conserved role during aging and in calorie restriction. | Inactivation of yeast Isw2 chromatin remodeling enzyme mimics longevity effect of calorie restriction via induction of genotoxic stress response.
Dang W, Sutphin GL, Dorsey JA, Otte GL, Cao K, Perry RM, Wanat JJ, Saviolaki D, Murakami CJ, Tsuchiyama S, Robison B, Gregory BD, Vermeulen M, Shiekhattar R, Johnson FB, Kennedy BK, Kaeberlein M, Berger SL., Free PMC Article | 06/20/2015 |
| Isw2 showed little evidence of nucleosome disassembly. | Comparison of the Isw1a, Isw1b, and Isw2 nucleosome disrupting activities.
Krajewski WA. | 02/8/2014 |
| Present evidence that a major targeting mechanism for the Isw2 chromatin remodeling enzyme to specific genomic loci is through sequence-specific transcription factor (TF)-dependent recruitment. | DNA looping facilitates targeting of a chromatin remodeling enzyme.
Yadon AN, Singh BN, Hampsey M, Tsukiyama T., Free PMC Article | 06/15/2013 |
| We find that remodelers( Isw1, Isw2, and Chd1 ) occupancy at NDRs and gene bodies is associated with nucleosome turnover and transcriptional elongation rate | ISWI and CHD chromatin remodelers bind promoters but act in gene bodies.
Zentner GE, Tsukiyama T, Henikoff S., Free PMC Article | 06/15/2013 |
| ISW2 has a conserved domain called SLIDE (SANT-like ISWI domain) that binds to extranucleosomal DNA ~19 base pairs from the edge of nucleosomes. | Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains.
Hota SK, Bhardwaj SK, Deindl S, Lin YC, Zhuang X, Bartholomew B., Free PMC Article | 04/6/2013 |
| Distinct ISWI-family members translocate nucleosomes with a similar stepping pattern maintained by the catalytic subunit of the enzyme. Nucleosome remodeling begins with a 7 bp step of DNA translocation followed by 3 bp subsequent steps toward the exit side of nucleosomes. | ISWI remodelers slide nucleosomes with coordinated multi-base-pair entry steps and single-base-pair exit steps.
Deindl S, Hwang WL, Hota SK, Blosser TR, Prasad P, Bartholomew B, Zhuang X., Free PMC Article | 03/30/2013 |
| The GST-tagged SET-domain polypeptide of human ALL-1 was assayed for binding to assembled mononucleosomes and nucleosome-dimer particles, either intact or remodeled with purified Isw2. | Remodeling of nucleosome-dimer particles with yIsw2 promotes their association with ALL-1 SET domain in vitro.
Krajewski WA, Vassiliev OL. | 07/28/2012 |
| Isw2 and Ino80 are both enriched at stalled replication forks and physically and specifically interact with a single-stranded DNA binding protein, replication protein A | ATP-dependent chromatin remodeling factors tune S phase checkpoint activity.
Au TJ, Rodriguez J, Vincent JA, Tsukiyama T., Free PMC Article | 12/24/2011 |
| Cbf1p binding to the upstream sites was required for the binding of the ISW2 chromatin-remodeling complex to the Ino2p-Ino4p-binding sites on the INO1 promoter. | Derepression of INO1 transcription requires cooperation between the Ino2p-Ino4p heterodimer and Cbf1p and recruitment of the ISW2 chromatin-remodeling complex.
Shetty A, Lopes JM., Free PMC Article | 03/12/2011 |
| yIsw2 is capable of translocating along single-stranded DNA in a reaction that is coupled to ATP hydrolysis. | Kinetic mechanism for single-stranded DNA binding and translocation by Saccharomyces cerevisiae Isw2.
Fischer CJ, Yamada K, Fitzgerald DJ., Free PMC Article | 01/21/2010 |
| Data show that the initial binding of ISW2 to extranucleosomal DNA, to the entry site, and near the dyad axis of the nucleosome is enhanced by ATP binding. | Conformational changes associated with template commitment in ATP-dependent chromatin remodeling by ISW2.
Gangaraju VK, Prasad P, Srour A, Kagalwala MN, Bartholomew B., Free PMC Article | 01/21/2010 |
| histone H4 tetra-acetylation was also found to reduce the activity of the Chd1 and Isw2 remodelling enzymes by reducing catalytic turnover | Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms.
Ferreira H, Flaus A, Owen-Hughes T., Free PMC Article | 01/21/2010 |
| The C-terminal domains of Isw2 are involved in anchoring the complex to nucleosomes through their interactions with linker DNA and that they facilitate the movement of DNA along the surface of nucleosomes. | Domain architecture of the catalytic subunit in the ISW2-nucleosome complex.
Dang W, Bartholomew B., Free PMC Article | 01/21/2010 |
| Isw2 is required for transcriptional silencing at the rDNA locus. | Isw2 regulates gene silencing at the ribosomal DNA locus in Saccharomyces cerevisiae.
Mueller JE, Li C, Bryk M., Free PMC Article | 01/21/2010 |
| Ssn6-Tup1 requires ISW2 complex to position nucleosomes. | Ssn6-Tup1 requires the ISW2 complex to position nucleosomes in Saccharomyces cerevisiae.
Zhang Z, Reese JC., Free PMC Article | 01/21/2010 |
| Mutant Isw2p (K215R), but not wild-type Isw2p, is a sensitive chromatin immunoprecipitation (ChIP) reagent for marking sites of Isw2 activity in vivo. | Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant.
Gelbart ME, Bachman N, Delrow J, Boeke JD, Tsukiyama T., Free PMC Article | 01/21/2010 |
| Linker DNA was found to be critical for the specific docking of ISW2 with nucleosomes as shown by mapping the physical contacts of ISW2 with nucleosomes at base-pair resolution. | Topography of the ISW2-nucleosome complex: insights into nucleosome spacing and chromatin remodeling.
Kagalwala MN, Glaus BJ, Dang W, Zofall M, Bartholomew B., Free PMC Article | 01/21/2010 |
| extranucleosomal DNA was required for nucleosome mobilization by ISW2 | Functional role of extranucleosomal DNA and the entry site of the nucleosome in chromatin remodeling by ISW2.
Zofall M, Persinger J, Bartholomew B., Free PMC Article | 01/21/2010 |
| histone H4 tail, in concert with a favorable length of extranucleosomal DNA, recruits and properly orients ISW2 onto the nucleosome for efficient nucleosome remodeling | Regulation of ISW2 by concerted action of histone H4 tail and extranucleosomal DNA.
Dang W, Kagalwala MN, Bartholomew B., Free PMC Article | 01/21/2010 |