| Two residues in the DNA binding site of Pif1 helicase are essential for nuclear functions but dispensable for mitochondrial respiratory growth. | Two residues in the DNA binding site of Pif1 helicase are essential for nuclear functions but dispensable for mitochondrial respiratory growth.
Gao J, Proffitt DR, Marecki JC, Protacio RU, Wahls WP, Byrd AK, Raney KD., Free PMC Article | 06/24/2024 |
| Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA. | Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA.
Chib S, Griffin WC, Gao J, Proffitt DR, Byrd AK, Raney KD., Free PMC Article | 12/20/2023 |
| Identification of the nuclear localization signal in the Saccharomyces cerevisiae Pif1 DNA helicase. | Identification of the nuclear localization signal in the Saccharomyces cerevisiae Pif1 DNA helicase.
Lee RS, Geronimo CL, Liu L, Twarowski JM, Malkova A, Zakian VA., Free PMC Article | 08/7/2023 |
| Single-molecule visualization of Pif1 helicase translocation on single-stranded DNA. | Single-molecule visualization of Pif1 helicase translocation on single-stranded DNA.
Mustafi M, Kwon Y, Sung P, Greene EC., Free PMC Article | 07/7/2023 |
| Pif1 family helicases promote mutation avoidance during DNA replication. | Pif1 family helicases promote mutation avoidance during DNA replication.
Zhou ZX, Follonier C, Lujan SA, Burkholder AB, Zakian VA, Kunkel TA., Free PMC Article | 03/10/2023 |
| Rad51-mediated interhomolog recombination during budding yeast meiosis is promoted by the meiotic recombination checkpoint and the conserved Pif1 helicase. | Rad51-mediated interhomolog recombination during budding yeast meiosis is promoted by the meiotic recombination checkpoint and the conserved Pif1 helicase.
Ziesel A, Weng Q, Ahuja JS, Bhattacharya A, Dutta R, Cheng E, Börner GV, Lichten M, Hollingsworth NM., Free PMC Article | 02/4/2023 |
| Identification of flexible Pif1-DNA interactions and their impacts on enzymatic activities. | Identification of flexible Pif1-DNA interactions and their impacts on enzymatic activities.
Li J, Ma J, Kumar V, Fu H, Xu C, Wang S, Jia Q, Fan Q, Xi X, Li M, Liu H, Lu Y., Free PMC Article | 12/17/2022 |
| Strand switching mechanism of Pif1 helicase induced by its collision with a G-quadruplex embedded in dsDNA. | Strand switching mechanism of Pif1 helicase induced by its collision with a G-quadruplex embedded in dsDNA.
Valle-Orero J, Rieu M, Tran PLT, Joubert A, Raj S, Allemand JF, Croquette V, Boulé JB., Free PMC Article | 11/19/2022 |
| Pif1 Activity is Modulated by DNA Sequence and Structure. | Pif1 Activity is Modulated by DNA Sequence and Structure.
Nickens DG, Bochman ML., Free PMC Article | 02/19/2022 |
| Dynamic regulation of Pif1 acetylation is crucial to the maintenance of genome stability. | Dynamic regulation of Pif1 acetylation is crucial to the maintenance of genome stability.
Ononye OE, Sausen CW, Bochman ML, Balakrishnan L., Free PMC Article | 07/10/2021 |
| The Pif1 helicase is actively inhibited during meiotic recombination which restrains gene conversion tract length. | The Pif1 helicase is actively inhibited during meiotic recombination which restrains gene conversion tract length.
Vernekar DV, Reginato G, Adam C, Ranjha L, Dingli F, Marsolier MC, Loew D, Guérois R, Llorente B, Cejka P, Borde V., Free PMC Article | 06/19/2021 |
| Lysine acetylation regulates the activity of nuclear Pif1. | Lysine acetylation regulates the activity of nuclear Pif1.
Ononye OE, Sausen CW, Balakrishnan L, Bochman ML., Free PMC Article | 03/13/2021 |
| Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family. | Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family.
Muellner J, Schmidt KH., Free PMC Article | 01/9/2021 |
| The unwinding of double-stranded DNA by Pif1 is dominated by highly processive and repetitive attempts of partial DNA opening. | Branched unwinding mechanism of the Pif1 family of DNA helicases.
Singh SP, Soranno A, Sparks MA, Galletto R., Free PMC Article | 04/18/2020 |
| Pif1DeltaN was still able to synergize with the Hrq1 helicase to inhibit telomerase activity in vitro, similar to full-length Pif1. These data impact our understanding of Pif1 helicase evolution and the roles of these enzymes in the maintenance of genome integrity. | The Biochemical Activities of the Saccharomyces cerevisiae Pif1 Helicase Are Regulated by Its N-Terminal Domain.
Nickens DG, Sausen CW, Bochman ML., Free PMC Article | 12/28/2019 |
| Data show that, In one type, DNA helicase PIF1 (ScPif1) moves along the ssDNA track with a rate of approximately 140 nt/s in 100 muM ATP, whereas in the other type, ScPif1 is immobilized to a fixed location of ssDNA and generates ssDNA loops against force. | Direct quantification of the translocation activities of Saccharomyces cerevisiae Pif1 helicase.
Lu C, Le S, Chen J, Byrd AK, Rhodes D, Raney KD, Yan J., Free PMC Article | 12/14/2019 |
| Complementary roles of Pif1 helicase and single stranded DNA binding proteins RPA and DNA polymerase subunits in stimulating DNA replication through G-quadruplexes have been demonstrated. | Complementary roles of Pif1 helicase and single stranded DNA binding proteins in stimulating DNA replication through G-quadruplexes.
Sparks MA, Singh SP, Burgers PM, Galletto R., Free PMC Article | 12/7/2019 |
| We showed that the Pif1 and Rrm3 DNA helicases promote efficient fork convergence and completion of DNA synthesis, even in the absence of type II topoisomerase. Furthermore, Rrm3 and Pif1 are also important for termination of plasmid DNA replication in vivo. | Pif1-Family Helicases Support Fork Convergence during DNA Replication Termination in Eukaryotes.
Deegan TD, Baxter J, Ortiz Bazán MÁ, Yeeles JTP, Labib KPM., Free PMC Article | 10/19/2019 |
| The structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase has been reported. | Insights into the structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase.
Lu KY, Chen WF, Rety S, Liu NN, Wu WQ, Dai YX, Li D, Ma HY, Dou SX, Xi XG., Free PMC Article | 07/6/2019 |
| Although certain single amino acid changes in the SM can be tolerated, the presence and sequence of the ScPif1 SM were essential for all tested in vivo functions. Consistent with the in vivo analyses, in vitro studies showed that the presence and sequence of the ScPif1 SM were critical for ATPase activity but not substrate binding | The signature motif of the Saccharomyces cerevisiae Pif1 DNA helicase is essential in vivo for mitochondrial and nuclear functions and in vitro for ATPase activity.
Geronimo CL, Singh SP, Galletto R, Zakian VA., Free PMC Article | 06/29/2019 |
| Pif1 is essential for efficient replisome progression through lagging strand G-quadruplex DNA secondary structures. | Pif1 is essential for efficient replisome progression through lagging strand G-quadruplex DNA secondary structures.
Dahan D, Tsirkas I, Dovrat D, Sparks MA, Singh SP, Galletto R, Aharoni A., Free PMC Article | 06/29/2019 |
| W Here we report that budding yeast Pif1, a helicase known to be involved in the resolution of complex DNA structures as well as the maturation of Okazaki fragments during replication, contributes to DNA damage bypass. We show that Pif1 expands regions of single-stranded DNA, so-called daughter-strand gaps, left behind the replication fork as a consequence of replisome re-priming | The helicase Pif1 functions in the template switching pathway of DNA damage bypass.
García-Rodríguez N, Wong RP, Ulrich HD., Free PMC Article | 06/29/2019 |
| highlights the formidable capability of Pif1 to resolve the majority of G4s in S. cerevisiae sequences, narrows the fractions of G4s that may be challenging for genomic stability, and provides a framework for understanding the influence of different G4s on genomic stability via their processing by Pif1 | DNA-unwinding activity of Saccharomyces cerevisiae Pif1 is modulated by thermal stability, folding conformation, and loop lengths of G-quadruplex DNA.
Wang L, Wang QM, Wang YR, Xi XG, Hou XM., Free PMC Article | 05/4/2019 |
| results suggest that Hrq1 and Pif1 interact and that their concerted activities ensure proper telomere length homeostasis in vivo | The Saccharomyces cerevisiae Hrq1 and Pif1 DNA helicases synergistically modulate telomerase activity in vitro.
Nickens DG, Rogers CM, Bochman ML., Free PMC Article | 04/13/2019 |
| PIF1 promotes centromere replication and segregation, but only in the absence of Rrm3. | Two Pif1 Family DNA Helicases Cooperate in Centromere Replication and Segregation in Saccharomyces cerevisiae.
Chen CF, Pohl TJ, Pott S, Zakian VA., Free PMC Article | 03/16/2019 |