| A sensor complements the steric gate when DNA polymerase discriminates ribonucleotides. | A sensor complements the steric gate when DNA polymerase ϵ discriminates ribonucleotides.
Parkash V, Kulkarni Y, Bylund GO, Osterman P, Kamerlin SCL, Johansson E., Free PMC Article | 11/18/2023 |
| DNA polymerase epsilon leading strand signature mutations result from defects in its proofreading activity. | DNA polymerase ε leading strand signature mutations result from defects in its proofreading activity.
Johnson RE, Prakash L, Prakash S., Free PMC Article | 08/4/2023 |
| The evolving tale of Pol2 function. | The evolving tale of Pol2 function.
Gallitto M, Zhang Z., Free PMC Article | 04/1/2023 |
| Enhanced polymerase activity permits efficient synthesis by cancer-associated DNA polymerase variants at low dNTP levels. | Enhanced polymerase activity permits efficient synthesis by cancer-associated DNA polymerase ϵ variants at low dNTP levels.
Barbari SR, Beach AK, Markgren JG, Parkash V, Moore EA, Johansson E, Shcherbakova PV., Free PMC Article | 08/20/2022 |
| Checkpoint-mediated DNA polymerase epsilon exonuclease activity curbing counteracts resection-driven fork collapse. | Checkpoint-mediated DNA polymerase ε exonuclease activity curbing counteracts resection-driven fork collapse.
Pellicanò G, Al Mamun M, Jurado-Santiago D, Villa-Hernández S, Yin X, Giannattasio M, Lanz MC, Smolka MB, Yeeles J, Shirahige K, García-Díaz M, Bermejo R., Free PMC Article | 07/31/2021 |
| Study show that, in Saccharomyces cerevisiae, the mutation rate increases synergistically when a Polepsilon nucleotide selectivity defect is combined with a Poldelta proofreading defect, demonstrating extrinsic proofreading of Polepsilon errors by Poldelta. Combining Poldelta nucleotide selectivity and Polepsilon proofreading defects produces no synergy, indicating that Polepsilon cannot correct errors made by Poldelta. | DNA polymerase δ proofreads errors made by DNA polymerase ε.
Bulock CR, Xing X, Shcherbakova PV., Free PMC Article | 07/18/2020 |
| Here we show that Rad53 drives the hyper-SUMOylation of Pol2, the catalytic subunit of DNA polymerase epsilon, principally following replication forks stalling induced by nucleotide depletion | The S phase checkpoint promotes the Smc5/6 complex dependent SUMOylation of Pol2, the catalytic subunit of DNA polymerase ε.
Winczura A, Appanah R, Tatham MH, Hay RT, De Piccoli G., Free PMC Article | 02/22/2020 |
| Our findings thus indicate a direct role for SUMO in Pol2-mediated DNA synthesis and a molecular basis for Smc5/6-mediated regulation of genome stability | Sumoylation of the DNA polymerase ε by the Smc5/6 complex contributes to DNA replication.
Meng X, Wei L, Peng XP, Zhao X., Free PMC Article | 02/22/2020 |
| DNA polymerase epsilon outcompetes double-stranded DNA for recruiting Ctf18-RFC to the replisome. | Structural Basis for the Recruitment of Ctf18-RFC to the Replisome.
Grabarczyk DB, Silkenat S, Kisker C. | 12/1/2018 |
| To dissect the Saccharomyces cerevisiae RNA polymerase II TL at individual-residue resolution, we quantitatively phenotyped nearly all TL single variants en masse.Our structural genetics approach incorporates structural and phenotypic data for high-resolution dissection of transcription mechanisms and their evolution, and is readily applicable to other essential yeast proteins. | High-Resolution Phenotypic Landscape of the RNA Polymerase II Trigger Loop.
Qiu C, Erinne OC, Dave JM, Cui P, Jin H, Muthukrishnan N, Tang LK, Babu SG, Lam KC, Vandeventer PJ, Strohner R, Van den Brulle J, Sze SH, Kaplan CD., Free PMC Article | 05/13/2017 |
| By detecting Pol2 and Msh2 dynamics within the same strain, we established that the mismatch recognition complex binds origins and spreads to adjacent regions with the replisome. | The Eukaryotic Mismatch Recognition Complexes Track with the Replisome during DNA Synthesis.
Haye JE, Gammie AE., Free PMC Article | 06/4/2016 |
| Study finds that wild-type Pol epsilon molecules cannot proofread errors created by proofreading-defective Pol epsilon molecules, whereas Pol delta can not only proofread errors created by proofreading-defective Pol delta molecules, but can also proofread errors created by Pol epsilon-defective molecules. | Replicative DNA polymerase δ but not ε proofreads errors in Cis and in Trans.
Flood CL, Rodriguez GP, Bao G, Shockley AH, Kow YW, Crouse GF., Free PMC Article | 12/19/2015 |
| dNTP pool levels correlate with Pol epsilon mutator severity | dNTP pool levels modulate mutator phenotypes of error-prone DNA polymerase ε variants.
Williams LN, Marjavaara L, Knowels GM, Schultz EM, Fox EJ, Chabes A, Herr AJ., Free PMC Article | 08/8/2015 |
| Data suggest that DNA-directed DNA polymerase Pols alpha and delta are the primary lagging-strand replicases and Pol epsilon is primarily a leading-strand replicase. | Heterogeneous polymerase fidelity and mismatch repair bias genome variation and composition.
Lujan SA, Clausen AR, Clark AB, MacAlpine HK, MacAlpine DM, Malc EP, Mieczkowski PA, Burkholder AB, Fargo DC, Gordenin DA, Kunkel TA., Free PMC Article | 07/25/2015 |
| Authors report the presence of a [Fe-S] cluster directly within the active polymerase domain of Pol2 (residues 1-1187). | An iron-sulfur cluster in the polymerase domain of yeast DNA polymerase ε.
Jain R, Vanamee ES, Dzikovski BG, Buku A, Johnson RE, Prakash L, Prakash S, Aggarwal AK., Free PMC Article | 04/19/2014 |
| Functional analysis of Bre1p, an E3 ligase for histone H2B ubiquitylation, in regulation of RNA polymerase II association with active genes and transcription in vivo | Functional analysis of Bre1p, an E3 ligase for histone H2B ubiquitylation, in regulation of RNA polymerase II association with active genes and transcription in vivo.
Sen R, Lahudkar S, Durairaj G, Bhaumik SR., Free PMC Article | 06/1/2013 |
| Labeling of DNA polymerase epsilon catalytic subunit with the nick-containing DNA repair intermediate indicates that the DNA polymerase is involved in DNA repair in yeast | Ddc1 checkpoint protein and DNA polymerase ɛ interact with nick-containing DNA repair intermediate in cell free extracts of Saccharomyces cerevisiae.
Sukhanova MV, D'Herin C, van der Kemp PA, Koval VV, Boiteux S, Lavrik OI. | 12/31/2011 |
| show that subunits of DNA polymerase epsilon (Pol epsilon) are required but that Mrc1, a polymerase epsilon-associated checkpoint protein, is opposing senescence | Polymerase epsilon is required to maintain replicative senescence.
Deshpande AM, Ivanova IG, Raykov V, Xue Y, Maringele L., Free PMC Article | 06/4/2011 |
| In unperturbed cells, Mrc1 interacts independently with both the N-terminal and C-terminal halves of Pol2 (Pol2N and Pol2C). | Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint.
Lou H, Komata M, Katou Y, Guan Z, Reis CC, Budd M, Shirahige K, Campbell JL., Free PMC Article | 01/21/2010 |
| Sir2 silences gene transcription by targeting the transition between RNA polymerase II initiation and elongation | Sir2 silences gene transcription by targeting the transition between RNA polymerase II initiation and elongation.
Gao L, Gross DS., Free PMC Article | 01/21/2010 |
| a mutation in the 3'-5' exonuclease domain of Pol2 dramatically reduces the frequency of end joins formed with initial 3' flaps. Thus, Pol2 performs a key 3' end-processing step in NHEJ. | Proofreading activity of DNA polymerase Pol2 mediates 3'-end processing during nonhomologous end joining in yeast.
Tseng SF, Gabriel A, Teng SC., Free PMC Article | 01/21/2010 |
| These data support a model of the replication fork wherein the leading and lagging strand templates are primarily copied by Pol epsilon and Pol delta, respectively. | Division of labor at the eukaryotic replication fork.
Nick McElhinny SA, Gordenin DA, Stith CM, Burgers PM, Kunkel TA., Free PMC Article | 01/21/2010 |
| findings reveal a pattern indicating that DNA polymerase epsilon participates in leading-strand DNA replication | Yeast DNA polymerase epsilon participates in leading-strand DNA replication.
Pursell ZF, Isoz I, Lundström EB, Johansson E, Kunkel TA., Free PMC Article | 01/21/2010 |
| The active site location occupied by Met644 in Pol epsilon is a key determinant of DNA replication fidelity. | Regulation of B family DNA polymerase fidelity by a conserved active site residue: characterization of M644W, M644L and M644F mutants of yeast DNA polymerase epsilon.
Pursell ZF, Isoz I, Lundström EB, Johansson E, Kunkel TA., Free PMC Article | 01/21/2010 |
| RNAPII elongation is stimulated by H2B-K123 ubiquitination independent of H3-K4 methy | H2B-K123 ubiquitination stimulates RNAPII elongation independent of H3-K4 methylation.
Shukla A, Bhaumik SR. | 01/21/2010 |