| Context-dependent function of the transcriptional regulator Rap1 in gene silencing and activation in Saccharomyces cerevisiae. | Context-dependent function of the transcriptional regulator Rap1 in gene silencing and activation in Saccharomyces cerevisiae.
Bondra ER, Rine J., Free PMC Article | 10/12/2023 |
| Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae. | Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae.
Rosas Bringas FR, Stinus S, de Zoeten P, Cohn M, Chang M., Free PMC Article | 03/5/2022 |
| Telomerase, the recombination machinery and Rap1 play redundant roles in yeast telomere protection. | Telomerase, the recombination machinery and Rap1 play redundant roles in yeast telomere protection.
Kabaha MM, Tzfati Y. | 07/10/2021 |
| Budding yeast Rap1, but not telomeric DNA, is inhibitory for multiple stages of DNA replication in vitro. | Budding yeast Rap1, but not telomeric DNA, is inhibitory for multiple stages of DNA replication in vitro.
Douglas ME, Diffley JFX., Free PMC Article | 06/26/2021 |
| Rap1-mediated nucleosome displacement can regulate gene expression in senescent cells without impacting the pace of senescence. | Rap1-mediated nucleosome displacement can regulate gene expression in senescent cells without impacting the pace of senescence.
Song S, Perez JV, Svitko W, Ricketts MD, Dean E, Schultz D, Marmorstein R, Johnson FB., Free PMC Article | 01/16/2021 |
| A Prion Epigenetic Switch Establishes an Active Chromatin State. | A Prion Epigenetic Switch Establishes an Active Chromatin State.
Harvey ZH, Chakravarty AK, Futia RA, Jarosz DF., Free PMC Article | 08/22/2020 |
| Structural basis of G-quadruplex DNA recognition by Rap1. | Structural basis of G-quadruplex DNA recognition by the yeast telomeric protein Rap1.
Traczyk A, Liew CW, Gill DJ, Rhodes D., Free PMC Article | 08/1/2020 |
| The rescue of fused telomeres results from a condensin- and Rap1-driven chromosome folding that favors fusion entrapment where abscission takes place. | Condensin-Mediated Chromosome Folding and Internal Telomeres Drive Dicentric Severing by Cytokinesis.
Guérin TM, Béneut C, Barinova N, López V, Lazar-Stefanita L, Deshayes A, Thierry A, Koszul R, Dubrana K, Marcand S. | 01/18/2020 |
| REVIEW: Rap1 confers promoter directionality by repressing divergent transcription | Transcribe this way: Rap1 confers promoter directionality by repressing divergent transcription.
Wu ACK, Van Werven FJ., Free PMC Article | 09/14/2019 |
| Rap1 has an essential role in the fidelity of transcription initiation and in the suppression of pervasive transcription. | General Regulatory Factors Control the Fidelity of Transcription by Restricting Non-coding and Ectopic Initiation.
Challal D, Barucco M, Kubik S, Feuerbach F, Candelli T, Geoffroy H, Benaksas C, Shore D, Libri D. | 06/1/2019 |
| Rap1, a sequence-specific transcription factor limits access of basal transcription machinery to regulatory elements and adjacent sequences that act as divergent cryptic promoters, thereby providing directionality toward productive transcription. | Repression of Divergent Noncoding Transcription by a Sequence-Specific Transcription Factor.
Wu ACK, Patel H, Chia M, Moretto F, Frith D, Snijders AP, van Werven FJ., Free PMC Article | 06/1/2019 |
| We present evidence that these gains occurred because Mcm1 shares a mechanism of transcriptional activation with an ancestral regulator of the ribosomal protein genes, Rap1. Specifically, we show that Mcm1 and Rap1 have the inherent ability to cooperatively activate transcription through contacts with the general transcription factor TFIID | Intrinsic cooperativity potentiates parallel cis-regulatory evolution.
Sorrells TR, Johnson AN, Howard CJ, Britton CS, Fowler KR, Feigerle JT, Weil PA, Johnson AD., Free PMC Article | 01/12/2019 |
| Rap1 (ScRap1) can either activate or repress transcription. This bimodal transcriptional activity has led to the widespread study of the mode of action of ScRap1. This review summarizes current knowledge about ScRap1, including its structure, mechanisms of transcription regulation, and biological functions, and the future directions in the field. Review. | The multifunctional transcription factor Rap1: a regulator of yeast physiology.
Azad GK, Tomar RS. | 12/23/2017 |
| Pif1, a 5'-3' helicase, not only stimulates the strand displacement activity of Pol delta but it also allows efficient replication through the Rap1 block, by removing bound Rap1 in front of the polymerase. | Pif1 removes a Rap1-dependent barrier to the strand displacement activity of DNA polymerase δ.
Koc KN, Singh SP, Stodola JL, Burgers PM, Galletto R., Free PMC Article | 06/10/2017 |
| that Rap1 contains an AD required for Rap1-dependent gene transcription. The Rap1(AS) variant will likely also be useful for studies of the functions of Rap1 in other biological pathways. | Identification of a transcriptional activation domain in yeast repressor activator protein 1 (Rap1) using an altered DNA-binding specificity variant.
Johnson AN, Weil PA., Free PMC Article | 06/3/2017 |
| the yKu-distal portion of telomeres is bound by Rap1, which in turn reduces the potential for yKu(Yku80 and Yku70 )to mediate NHEJ. These results thus propose a solution to a long-standing conundrum, namely how to accommodate the apparently conflicting functions of Ku on telomeres. | Ku Binding on Telomeres Occurs at Sites Distal from the Physical Chromosome Ends.
Larcher MV, Pasquier E, MacDonald RS, Wellinger RJ., Free PMC Article | 05/13/2017 |
| Rap1 contributes to telomere homeostasis by promoting chromosome breakage. | Binding of Multiple Rap1 Proteins Stimulates Chromosome Breakage Induction during DNA Replication.
Goto GH, Zencir S, Hirano Y, Ogi H, Ivessa A, Sugimoto K., Free PMC Article | 05/14/2016 |
| found that all arrangements of Rap1 sites tested are represented within the telomeric sequence and our data suggest that at telomeres Rap1 might form a nucleoprotein complex with a heterogeneous distribution of bound states | Alternative arrangements of telomeric recognition sites regulate the binding mode of the DNA-binding domain of yeast Rap1.
Feldmann EA, Koc KN, Galletto R., Free PMC Article | 11/21/2015 |
| Heterozygous diploid knockout strains for FHL1, RAP1, REB1, and MCM1 genes showed significantly short lifespan. | Transcription factor genes essential for cell proliferation and replicative lifespan in budding yeast.
Kamei Y, Tai A, Dakeyama S, Yamamoto K, Inoue Y, Kishimoto Y, Ohara H, Mukai Y. | 09/12/2015 |
| Rap1 C-terminal (RCT) domain of yeast Rap1 modulate access to different DNA binding modes | The wrapping loop and Rap1 C-terminal (RCT) domain of yeast Rap1 modulate access to different DNA binding modes.
Feldmann EA, De Bona P, Galletto R., Free PMC Article | 07/25/2015 |
| findings suggest a connection between Rap1p and cell wall homeostasis. | The transcription factor Rap1p is required for tolerance to cell-wall perturbing agents and for cell-wall maintenance in Saccharomyces cerevisiae.
Azad GK, Singh V, Baranwal S, Thakare MJ, Tomar RS. | 02/21/2015 |
| while Rap1 DNA-binding domain forms a high-affinity 1:1 complex with its DNA recognition site, it can also form lower-affinity complexes with higher stoichiometries on DNA | The DNA-binding domain of yeast Rap1 interacts with double-stranded DNA in multiple binding modes.
Feldmann EA, Galletto R., Free PMC Article | 02/14/2015 |
| Rap1 relocalization is thus a novel mechanism connecting DNA damage responses (DDRs) at telomeres to global changes in chromatin and gene expression while driving the pace of senescence | Rap1 relocalization contributes to the chromatin-mediated gene expression profile and pace of cell senescence.
Platt JM, Ryvkin P, Wanat JJ, Donahue G, Ricketts MD, Barrett SP, Waters HJ, Song S, Chavez A, Abdallah KO, Master SR, Wang LS, Johnson FB., Free PMC Article | 08/31/2013 |
| The X-ray structures of Rif1 and Rif2 bound to the Rap1 C-terminal domain and that of the Rif1 C terminus are presented. Both Rif1 and Rif2 have separable and independent Rap1-binding epitopes, allowing Rap1 binding over large distances (42-110 A). | Rif1 and Rif2 shape telomere function and architecture through multivalent Rap1 interactions.
Shi T, Bunker RD, Mattarocci S, Ribeyre C, Faty M, Gut H, Scrima A, Rass U, Rubin SM, Shore D, Thomä NH. | 08/31/2013 |
| Rap1 may cause permanganate hypersensitivity of DNA by forming a pocket between the reactive cytosine and Arg580, driving the permanganate ion towards the C5-C6 bond of the cytosine. | Effect of Rap1 binding on DNA distortion and potassium permanganate hypersensitivity.
Le Bihan YV, Matot B, Pietrement O, Giraud-Panis MJ, Gasparini S, Le Cam E, Gilson E, Sclavi B, Miron S, Le Du MH. | 08/3/2013 |