| Experimental exchange of paralogous domains in the MLH family provides evidence of sub-functionalization after gene duplication. | Experimental exchange of paralogous domains in the MLH family provides evidence of sub-functionalization after gene duplication.
Furman CM, Elbashir R, Pannafino G, Clark NL, Alani E., Free PMC Article | 11/19/2022 |
| Handcuffing intrinsically disordered regions in Mlh1-Pms1 disrupts mismatch repair. | Handcuffing intrinsically disordered regions in Mlh1-Pms1 disrupts mismatch repair.
Furman CM, Wang TY, Zhao Q, Yugandhar K, Yu H, Alani E., Free PMC Article | 11/6/2021 |
| Results provide evidence that the PMS1 gene encoding one of the crucial components of the mismatch repair, is required for transcriptional repression at silent mating-type loci and telomeres. | Yeast mismatch repair components are required for stable inheritance of gene silencing.
Liu Q, Zhu X, Lindström M, Shi Y, Zheng J, Hao X, Gustafsson CM, Liu B., Free PMC Article | 08/1/2020 |
| Mlh1-Pms1 complexes with shorter IDRs that disrupt MMR retain wild-type DNA binding affinity but are impaired for diffusion on both naked and nucleosome-coated DNA. Moreover, the IDRs also regulate the adenosine triphosphate hydrolysis and nuclease activities that are encoded in the structured N- and C-terminal domains of the complex | Intrinsically disordered regions regulate both catalytic and non-catalytic activities of the MutLα mismatch repair complex.
Kim Y, Furman CM, Manhart CM, Alani E, Finkelstein IJ., Free PMC Article | 08/31/2019 |
| Genotyping analysis indicated that MLH1-PMS1 incompatibility was the major driver of mutation rate in the isolates. The variation in the mutation rate of incompatible spore clones could be due to background suppressors and enhancers, as well as aneuploidy seen in the spore clones. | Incompatibilities in Mismatch Repair Genes MLH1-PMS1 Contribute to a Wide Range of Mutation Rates in Human Isolates of Baker's Yeast.
Raghavan V, Bui DT, Al-Sweel N, Friedrich A, Schacherer J, Aquadro CF, Alani E., Free PMC Article | 03/2/2019 |
| Two mutants of MutLalpha, pms1-G882E and pms1-H888R, repair deletion mispairs but not insertion mispairs. | Different roles of eukaryotic MutS and MutL complexes in repair of small insertion and deletion loops in yeast.
Romanova NV, Crouse GF., Free PMC Article | 02/21/2015 |
| a comparative study of Msh2-Msh3 and Msh2-Msh6 for mispair binding, sliding clamp formation, and Mlh1-Pms1 recruitment | Mispair-specific recruitment of the Mlh1-Pms1 complex identifies repair substrates of the Saccharomyces cerevisiae Msh2-Msh3 complex.
Srivatsan A, Bowen N, Kolodner RD., Free PMC Article | 05/24/2014 |
| The unstructured linker arms of Mlh1-Pms1 are important for interactions with DNA during mismatch repair | The unstructured linker arms of Mlh1-Pms1 are important for interactions with DNA during mismatch repair.
Plys AJ, Rogacheva MV, Greene EC, Alani E., Free PMC Article | 11/17/2012 |
| Functional residues on the surface of the N-terminal domain of yeast Pms1 | Functional residues on the surface of the N-terminal domain of yeast Pms1.
Arana ME, Holmes SF, Fortune JM, Moon AF, Pedersen LC, Kunkel TA., Free PMC Article | 07/5/2010 |
| Adenine nucleotides induce large asymmetric conformational changes in full-length yeast MutL alpha. These changes are associated with significant increases in secondary structure. | Direct visualization of asymmetric adenine-nucleotide-induced conformational changes in MutL alpha.
Sacho EJ, Kadyrov FA, Modrich P, Kunkel TA, Erie DA., Free PMC Article | 01/21/2010 |
| genetic analysis of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 and MLH1-PMS1 complexes with DNA | Analysis of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 and MLH1-PMS1 complexes with DNA using a reversible DNA end-blocking system.
Mendillo ML, Mazur DJ, Kolodner RD. | 01/21/2010 |
| Saccharomyces cerevisiae strains bearing the S288c-strain-derived MLH1 gene and the SK1-strain-derived PMS1 gene displayed elevated mutation rates that conferred a long-term fitness cost | Negative epistasis between natural variants of the Saccharomyces cerevisiae MLH1 and PMS1 genes results in a defect in mismatch repair.
Heck JA, Argueso JL, Gemici Z, Reeves RG, Bernard A, Aquadro CF, Alani E., Free PMC Article | 01/21/2010 |
| Suggested that the inherent inefficiency of primer strand loop repair involves Pms1 that functions downstream of mismatch recognition. The findings reinforce the current view that during mutation avoidance, MMR is associated with the replication apparatus | Novel PMS1 alleles preferentially affect the repair of primer strand loops during DNA replication.
Erdeniz N, Dudley S, Gealy R, Jinks-Robertson S, Liskay RM., Free PMC Article | 01/21/2010 |