| The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation. | The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.
Kornakov N, Möllers B, Westermann S., Free PMC Article | 04/3/2021 |
| The kinesin-14 motor protein complex (Cik1-Kar3) cooperates with chromatin remodellers to mediate interactions between subtelomeric double-strand breaks and the Nup84 nuclear pore complex to ensure cell survival via break-induced replication. | Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process.
Chung DK, Chan JN, Strecker J, Zhang W, Ebrahimi-Ardebili S, Lu T, Abraham KJ, Durocher D, Mekhail K. | 05/14/2016 |
| findings provide an example for a non-conventional translocation mechanism and can explain how Kar3 substitutes for key functions of Dynein in the yeast nucleus | Non-catalytic motor domains enable processive movement and functional diversification of the kinesin-14 Kar3.
Mieck C, Molodtsov MI, Drzewicka K, van der Vaart B, Litos G, Schmauss G, Vaziri A, Westermann S., Free PMC Article | 12/5/2015 |
| Cryoelectron microscopy generated 3-D structure of Kar3Cik1 complexed to microtubules in various nucleotide states. | Common mechanistic themes for the powerstroke of kinesin-14 motors.
Gonzalez MA, Cope J, Rank KC, Chen CJ, Tittmann P, Rayment I, Gilbert SP, Hoenger A., Free PMC Article | 06/28/2014 |
| Kar3, with the help of its light chain, Cik1, is anchored during mating to the SPB component Spc72 that also serves as a nucleator and anchor for microtubules via their minus ends. | Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy.
Gibeaux R, Politi AZ, Nédélec F, Antony C, Knop M., Free PMC Article | 03/23/2013 |
| Neck rotation and neck mimic docking in Vik1 and Cik1 is a structural mechanism for communication with Kar3. | Neck rotation and neck mimic docking in the noncatalytic Kar3-associated protein Vik1.
Duan D, Jia Z, Joshi M, Brunton J, Chan M, Drew D, Davis D, Allingham JS., Free PMC Article | 03/2/2013 |
| Rapid ATP binding to Kar3 is associated with rotation of the coiled-coil stalk, and the postpowerstroke ATP hydrolysis is independent of Vik1. | The ATPase pathway that drives the kinesin-14 Kar3Vik1 powerstroke.
Chen CJ, Porche K, Rayment I, Gilbert SP., Free PMC Article | 01/12/2013 |
| show through structural determination of a C-terminal heterodimeric Kar3Vik1, electron microscopy, equilibrium binding, and motility that at the start of the cycle, Kar3Vik1 binds to or occludes two alphabeta-tubulin subunits on adjacent protofilaments | Kar3Vik1, a member of the kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern.
Rank KC, Chen CJ, Cope J, Porche K, Hoenger A, Gilbert SP, Rayment I., Free PMC Article | 09/1/2012 |
| we found that Cik1 mediates the association of Kar3 with kinetochores and this association only occurs before chromosome bipolar attachment. | Loss of function of the Cik1/Kar3 motor complex results in chromosomes with syntelic attachment that are sensed by the tension checkpoint.
Jin F, Liu H, Li P, Yu HG, Wang Y., Free PMC Article | 06/30/2012 |
| a model in which Kar3Cik1 interacts with the MT lattice through an alternating cycle of Cik1 MT collision followed by Kar3 MT binding with head-head communication between Kar3 and Cik1 modulated by the Kar3 nucleotide state and intramolecular strain | Kinesin Kar3Cik1 ATPase pathway for microtubule cross-linking.
Chen CJ, Rayment I, Gilbert SP., Free PMC Article | 10/22/2011 |
| Gpa1 serves as a positional determinant for Kar3-bound microtubule plus ends during mating. | The mating-specific Galpha interacts with a kinesin-14 and regulates pheromone-induced nuclear migration in budding yeast.
Zaichick SV, Metodiev MV, Nelson SA, Durbrovskyi O, Draper E, Cooper JA, Stone DE., Free PMC Article | 01/21/2010 |
| Kar3p contributes to spindle stability by cross-linking spindle microtubules. | The microtubule-based motor Kar3 and plus end-binding protein Bim1 provide structural support for the anaphase spindle.
Gardner MK, Haase J, Mythreye K, Molk JN, Anderson M, Joglekar AP, O'Toole ET, Winey M, Salmon ED, Odde DJ, Bloom K., Free PMC Article | 01/21/2010 |
| Vik1 binds more tightly to microtubules than Kar3 and facilitates cooperative microtubule decoration by Kar3/Vik1 heterodimers, and yet allows motility. | Vik1 modulates microtubule-Kar3 interactions through a motor domain that lacks an active site.
Allingham JS, Sproul LR, Rayment I, Gilbert SP., Free PMC Article | 01/21/2010 |
| the kar3 meiotic arrest may be mediated by the spindle checkpoint | Analysis of the kar3 meiotic arrest in Saccharomyces cerevisiae.
Shanks RM, Bascom-Slack C, Dawson DS. | 01/21/2010 |
| In Kar3, the extended alpha-helical domain NH2-terminal to the catalytic core provides the structural transitions in response to the ATPase cycle that are critical for motility; dimerization is not specifically required | Mechanistic analysis of the Saccharomyces cerevisiae kinesin Kar3.
Mackey AT, Sproul LR, Sontag CA, Satterwhite LL, Correia JJ, Gilbert SP., Free PMC Article | 01/21/2010 |
| structural changes in Kar3 upon dimerization with Cik1 alter the motor velocity and likely regulate Kar3 activity in vivo | Kar3 interaction with Cik1 alters motor structure and function.
Chu HM, Yun M, Anderson DE, Sage H, Park HW, Endow SA., Free PMC Article | 01/21/2010 |
| Kinetochores slide along the microtubule lateral surface, which is mainly and probably exclusively driven by Kar3. | Molecular mechanisms of microtubule-dependent kinetochore transport toward spindle poles.
Tanaka K, Kitamura E, Kitamura Y, Tanaka TU., Free PMC Article | 01/21/2010 |