| The yeast Aft1 transcription factor activates ribonucleotide reductase catalytic subunit RNR1 in response to iron deficiency. | The yeast Aft1 transcription factor activates ribonucleotide reductase catalytic subunit RNR1 in response to iron deficiency.
Ros-Carrero C, Ramos-Alonso L, Romero AM, Bañó MC, Martínez-Pastor MT, Puig S. | 10/24/2020 |
| We identified 24 rnr1 mutant alleles resulting in diverse mutator phenotypes linked in most cases to imbalanced dNTPs. Among the identified rnr1 alleles the strongest mutators presented a dNTP imbalance in which three out of the four dNTPs were elevated (dCTP, dTTP and dGTP), particularly if dGTP levels were highly increased. | A genetic screen pinpoints ribonucleotide reductase residues that sustain dNTP homeostasis and specifies a highly mutagenic type of dNTP imbalance.
Schmidt TT, Sharma S, Reyes GX, Gries K, Gross M, Zhao B, Yuan JH, Wade R, Chabes A, Hombauer H., Free PMC Article | 08/31/2019 |
| Fhl1p regulates RNR1 gene transcription to maintain dNTP levels, thus modulating longevity by protection against replication stress | The forkhead-like transcription factor (Fhl1p) maintains yeast replicative lifespan by regulating ribonucleotide reductase 1 (RNR1) gene transcription.
Tai A, Kamei Y, Mukai Y. | 09/23/2017 |
| Multiple turnover kinetics show that Sml1 inhibition of dGTP/ADP- and ATP/CDP-bound ScRR follows a mixed inhibition mechanism. However, Sml1 cooperatively binds to the ES complex in the dGTP/ADP form, whereas with ATP/CDP, Sml1 binds weakly and noncooperatively | Inhibition of yeast ribonucleotide reductase by Sml1 depends on the allosteric state of the enzyme.
Misko TA, Wijerathna SR, Radivoyevitch T, Berdis AJ, Ahmad MF, Harris ME, Dealwis CG., Free PMC Article | 04/29/2017 |
| there is a directed orientation of the RNR beta and beta' C-terminal tails relative to alpha within the holoenzyme consistent with a docking model of the two subunits and argue against RT across the beta beta' interface | Investigation of in vivo roles of the C-terminal tails of the small subunit (ββ') of Saccharomyces cerevisiae ribonucleotide reductase: contribution to cofactor formation and intersubunit association within the active holoenzyme.
Zhang Y, An X, Stubbe J, Huang M., Free PMC Article | 08/10/2013 |
| The data reveaedl that the arginine 293 and glutamine 288 residues of ribonucleotide reductase 1 are crucial in facilitating ADP and CDP substrate selection. | Role of arginine 293 and glutamine 288 in communication between catalytic and allosteric sites in yeast ribonucleotide reductase.
Ahmad MF, Kaushal PS, Wan Q, Wijerathna SR, An X, Huang M, Dealwis CG., Free PMC Article | 08/4/2012 |
| defects in autophagy result in an increase in soluble Rnr1 protein levels and a DNA damage phenotype. | Autophagy-dependent regulation of the DNA damage response protein ribonucleotide reductase 1.
Dyavaiah M, Rooney JP, Chittur SV, Lin Q, Begley TJ. | 08/13/2011 |
| Data show that Rnr1 transcription requires protein bound to a particular site and either five different sites or one additional sites. | Reverse engineering the yeast RNR1 transcriptional control system.
Mao G, Brody JP., Free PMC Article | 04/30/2011 |
| x-ray structures upon binding to gemcitabine diphosphate | Structures of eukaryotic ribonucleotide reductase I define gemcitabine diphosphate binding and subunit assembly.
Xu H, Faber C, Uchiki T, Racca J, Dealwis C., Free PMC Article | 01/21/2010 |
| The structures of the apo-, AMPPNP only-, AMPPNP-CDP-, AMPPNP-UDP-, dGTP-ADP- and TTP-GDP-bound complexes give insight into substrate and effector binding and specificity cross-talk. | Structures of eukaryotic ribonucleotide reductase I provide insights into dNTP regulation.
Xu H, Faber C, Uchiki T, Fairman JW, Racca J, Dealwis C., Free PMC Article | 01/21/2010 |
| Sml1 competes with RNR1 C-terminal domain for association with RNR1 N-terminal domain to hinder the accessibility of the CX2C motif to the active site for RNR1 regeneration. | Role of the C terminus of the ribonucleotide reductase large subunit in enzyme regeneration and its inhibition by Sml1.
Zhang Z, Yang K, Chen CC, Feser J, Huang M., Free PMC Article | 01/21/2010 |