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| Status |
Public on Jun 01, 2019 |
| Title |
Transcriptome Analyses of LncRNA Metabolic Regulators from High Fat Diet Induced T2DM Mouse Adipose Tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To search long noncoding RNAs (lncRNAs) which regulating energy metabolism in high fat diet induced T2DM mouse, we performed transcriptome analyses to simultaneously profile mRNAs and lncRNAs in epididymal adipose tissue in normal and high fat diet induced mouse. Combining genome-wide screens, bioinformatics function predictions, and cell-based analyses, we developed an integrative roadmap to identify lncRNA metabolic regulators in epididymal adipose tissue under high fat diet induced T2DM mouse.
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| Overall design |
To identify regulated lncRNAs in adipose tissues and to establish an inclusive data set to predict lncRNA functions in energy metabolism on the basis of their correlation with mRNAs, we simultaneously profiled all mRNAs and lncRNAs in epididymal adipose tissue in mice（C57BL/6J）under long-term (12 weeks) HFD (high fat diet) feeding, a widely used model mimicking dietary obesity in humans，the ND（normal diet） feeding animals as control. We performed 6 microarray analyses for each group of three mice for RNA extraction and hybridization on Affymetrix microarrays .
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| Contributor(s) |
Yao Z, Ren P |
| Citation(s) |
31464075, 36333974 |
| |
| Submission date |
Jun 14, 2017 |
| Last update date |
Jan 13, 2023 |
| Contact name |
yao zhenyu |
| E-mail(s) |
[email protected]
|
| Organization name |
Shenzhen institutes of advanced technology, chinese academy of science
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| Department |
Institute of biomedical and Health Engineering
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| Street address |
No. 1068 xueyuan road Nanshan District Shenzhen
|
| City |
Shenzhen |
| State/province |
Guang dong province |
| ZIP/Postal code |
518055 |
| Country |
China |
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| Platforms (1) |
| GPL20258 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CDF version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA390520 |
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