|
| Status |
Public on Jan 24, 2008 |
| Title |
Inhibition of phosphatidylinositol 3-kinase signaling in proliferating human T98G cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Continuous PI 3-kinase signaling is required for cell proliferation and survival, but the gene expression program controlled by PI 3-kinase in proliferating cells has not been elucidated. We used microarray analyses to characterize the changes in gene expression resulting from inhibition of PI 3-kinase in actively proliferating cells. Following 2 and 4 hours of PI 3-kinase inhibition, the time around which apoptosis initiated, 32 genes were significantly up-regulated and 53 down-regulated. After 8 hours of inhibition, the total number of expression changes increased to over 200. The genes regulated by inhibition of PI 3-kinase in proliferating cells were distinct from genes induced by growth factor stimulation of quiescent cells and included key regulators of cell proliferation and programmed cell death.
Keywords: time course
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| |
|
| Overall design |
Transcriptional profiling time course of proliferating human T98G cells following LY294002 treatment. RNA was harvested at 2, 4 and 8 hr following LY294002 treatment. Two-color microarrays with dye-swap; 3 biological replicates per condition, independently grown and harvested. One replicate per array.
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| |
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| Contributor(s) |
Terragni J, Graham JR, Adams KW, Schaffer ME, Cooper GM |
| Citation(s) |
18226221 |
| |
| Submission date |
Jan 21, 2008 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Geoffrey M Cooper |
| Organization name |
Boston University
|
| Department |
Biology
|
| Lab |
|
| Street address |
5 Cummington St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5444 |
Operon Human V2; print layout 1 |
|
| Samples (18)
|
| GSM258007 |
T98G 2 hr LY294002 vs Untreated Cy5-Cy3 rep 1 |
| GSM258008 |
T98G 2 hr LY294002 vs Untreated Cy3-Cy5 rep 1 |
| GSM258009 |
T98G 2 hr LY294002 vs Untreated Cy5-Cy3 rep 2 |
| GSM258010 |
T98G 2 hr LY294002 vs Untreated Cy3-Cy5 rep 2 |
| GSM258011 |
T98G 2 hr LY294002 vs Untreated Cy5-Cy3 rep 3 |
| GSM258012 |
T98G 2 hr LY294002 vs Untreated Cy3-Cy5 rep 3 |
| GSM258013 |
T98G 4 hr LY294002 vs Untreated Cy5-Cy3 rep 1 |
| GSM258014 |
T98G 4 hr LY294002 vs Untreated Cy3-Cy5 rep 1 |
| GSM258015 |
T98G 4 hr LY294002 vs Untreated Cy5-Cy3 rep 2 |
| GSM258016 |
T98G 4 hr LY294002 vs Untreated Cy3-Cy5 rep 2 |
| GSM258017 |
T98G 4 hr LY294002 vs Untreated Cy5-Cy3 rep 3 |
| GSM258018 |
T98G 4 hr LY294002 vs Untreated Cy3-Cy5 rep 3 |
| GSM258019 |
T98G 8 hr LY294002 vs Untreated Cy5-Cy3 rep 1 |
| GSM258020 |
T98G 8 hr LY294002 vs Untreated Cy3-Cy5 rep 1 |
| GSM258021 |
T98G 8 hr LY294002 vs Untreated Cy5-Cy3 rep 2 |
| GSM258022 |
T98G 8 hr LY294002 vs Untreated Cy3-Cy5 rep 2 |
| GSM258023 |
T98G 8 hr LY294002 vs Untreated Cy5-Cy3 rep 3 |
| GSM258024 |
T98G 8 hr LY294002 vs Untreated Cy3-Cy5 rep 3 |
|
| Relations |
| BioProject |
PRJNA108283 |
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