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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 29, 2018 |
| Title |
Translation cooperation of FGF and WNT signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cooperation between FGF and WNT signaling is well documented in normal development, stem cell biology and cancer progression. However, the molecular mechanisms underlying this cooperativity remain poorly understood. In this study, we employed an inducible FGFR1 to interrogate the dynamics of RNA, ribosome occupancy and protein expression as a function of FGFR signaling in the mouse mammary gland with constitutive WNT hyperactivation. We demonstrate that FGFR signaling increases the translation efficiency of WNT signaling components with structured 5’ UTRs harboring a (CGG)4 motif that can potentially form G-quadruplex structures, and that tumorigenesis driven by this mechanism is vulnerable to inhibition of eIF4A, a RNA helicase important in translation initiation. This study also provides novel insights into the contribution of RNA levels and translational regulation to protein expression in pre-malignant mammary epithelial cells dynamically responding to FGFR signaling.
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| Overall design |
RNA and ribosome footprint profiles of WNT-hyperactive mammary epithelial cells from 4-week old mice with and without FGFR1 signaling overactivation in the mammary glands were generated by deep sequencing, in triplicate, using the HiSeq 2500 platform
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| Contributor(s) |
Nguyen TM, Kabotyanski EB, Dou Y, Reineke LC, Zhang P, Zhang X, Malovannaya A, Jung SY, Mo Q, Roarty K, Chen Y, Zhang B, Neilson J, Lloyd RE, Perou CM, Ellis MJ, Rosen JM |
| Citation(s) |
29844125 |
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| Submission date |
Dec 11, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jeffrey M Rosen |
| Organization name |
Baylor College of Medicine
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| Department |
Molecular and Cellular Biology
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| Street address |
1 Baylor Plaza
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| City |
HOUSTON |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA421945 |
| SRA |
SRP126508 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE107926_raw_count.csv.gz |
204.4 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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