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| Status |
Public on Jun 24, 2008 |
| Title |
The MILE (Microarray Innovations In LEukemia) study pre-phase |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
An international standardization program towards the application of gene expression profiling in routine leukaemia diagnostics: The MILE study pre-phase.
A total of 11 laboratories from three continents performed 204 analyses using a whole-genome expression microarray.
In an effort to standardize the microarray procedure, each participating centre was equipped with identical hardware, software, and reagents. Designated study operators received intensive individual training on the sample preparation protocol.
Keywords: Standardization and reproducibility of microarray analysis
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| Overall design |
This study is part of the MILE Study (Microarray Innovations In LEukemia) program, headed by the European Leukaemia Network (ELN) and sponsored by Roche Molecular Systems, Inc.
Each laboratory prepared two separate cell line total RNA samples, as well as three replicate leukaemia patient lysates in two distinct project stages: (i) a five-day course of protocol training, and (ii) independent proficiency testing.
Using unsupervised, supervised, and r² correlation analyses we demonstrated that microarray analysis can be performed not only with remarkably high intra-laboratory reproducibility but also with comparable quality and reliability between eleven distinct laboratories.
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| Citation(s) |
18573112 |
| |
| Submission date |
Apr 10, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Rachel Li |
| E-mail(s) |
[email protected]
|
| Phone |
925 730-8136
|
| Organization name |
Roche
|
| Street address |
4300 Hacienda Dr.
|
| City |
Pleasanton |
| State/province |
CA |
| ZIP/Postal code |
94588 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (204)
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| Relations |
| BioProject |
PRJNA106923 |
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