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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 22, 2008 |
| Title |
Dicer-null cell lines vs. wt ES cells and Dicer-null cell lines vs. Dicer-null cells overexpressing Dnmt1 or Dnmt3a/3b |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dicer initiates RNA interference by generating small RNAs involved in various silencing pathways. Dicer participates in centromeric silencing, but its role in the epigenetic regulation of other chromatin domains has not been explored. Here we show that Dicer1 deficiency in Mus musculus leads to decreased DNA methylation, concomitant with increased telomere recombination and telomere elongation. These DNA-methylation defects correlate with decreased expression of Dnmt1, Dnmt3a and Dnmt3b DNA methyltransferases (Dnmts), and methylation levels can be recovered by their overexpression. We identify the retinoblastoma-like 2 protein (Rbl2) as responsible for decreased Dnmt expression in Dicer1-null cells, suggesting the existence of Dicer-dependent small RNAs that target Rbl2.
We identify the miR-290 cluster as being downregulated in Dicer1-deficient cells and show that it silences Rbl2, thereby controlling Dnmt expression. These results identify a pathway by which miR-290 directly regulates Rbl2-dependent Dnmt expression, indirectly affecting telomere-length homeostasis.
Keywords: Cell type comparison
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| Overall design |
Two-condition experiments. 6 comparisons. One dye-swap technical replicate for all samples. 2-color platform.
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| Contributor(s) |
Benetti R, Gonzalo S, Jaco I, Muñoz P, González S, Schoeftner S, Murchison E, Andl T, Chenn T, Klatt P, Li E, Serrano M, Millar S, Hannon G, Blasco MA |
| Citation(s) |
18311151 |
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| Submission date |
Apr 21, 2008 |
| Last update date |
May 10, 2018 |
| Contact name |
Roberta Benetti |
| E-mail(s) |
[email protected]
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| Organization name |
CNIO
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| Street address |
Calle Melchor Fernandez Almagro
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| City |
Madrid |
| ZIP/Postal code |
28003 |
| Country |
Spain |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (12)
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| GSM283107 |
Dicer1-null 27G5 cells overexpressing Dnmt1 versus Dicer1-null 27G5 cells 1 |
| GSM283108 |
Dicer1-null 27G5 cells overexpressing Dnmt1 versus Dicer1-null 27G5 cells 2 |
| GSM283109 |
Dicer1-null 27G5 cells overexpressing Dnmt3a,3b versus Dicer1-null 27G5 cells 1 |
| GSM283110 |
Dicer1-null 27G5 cells overexpressing Dnmt3a,3b versus Dicer1-null 27G5 cells 2 |
| GSM283111 |
Dicer1-null 27H10 cells overexpressing Dnmt1 versus Dicer1-null 27H10 cells 1 |
| GSM283112 |
Dicer1-null 27H10 cells overexpressing Dnmt1 versus Dicer1-null 27H10 cells 2 |
| GSM283113 |
Dicer1-null 27H10 overexpressing Dnmt3a,3b versus Dicer1-null 27H10 cells 1 |
| GSM283114 |
Dicer1-null 27H10 overexpressing Dnmt3a,3b versus Dicer1-null 27H10 cells 2 |
| GSM283115 |
Dicer1-null 27G5 versus wild-type ES cells 1 |
| GSM283116 |
Dicer1-null 27H10 versus wild-type ES cells 1 |
| GSM283117 |
Dicer1-null 27G5 versus wild-type ES cells 2 |
| GSM283118 |
Dicer1-null 27H10 versus wild-type ES cells 2 |
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| Relations |
| BioProject |
PRJNA106723 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11229_RAW.tar |
187.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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