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| Status |
Public on Apr 23, 2021 |
| Title |
Genome-wide DNA methylation maps of Decitabine and/or Cisplatin treated bladder cancer cells. |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
It is well established that low-dose Decitabine treatment can sustain long-term antitumor effects by targeting DNA methylation and altering gene expression32. We performed methylated DNA immunoprecipitation (MEDIP) followed by next-generation sequencing in 5637 cells. Daily Decitabine treatment with 100 nM for 72 hr (with or without 100ng/ml cisplatin treatment) reduced the methylation level at CpG-islands in the genome of 5637 cells . However, this low-dose DEC treatment did not cause a significant reduction of DNA methylation in non-CpG islands regions.Our study represents the first detailed analysis of DNA methylome generated by RNA-seq technology in bladder cancer cells treated with low-dose decitabine. This study help to reveal the direct target genes of decitabine in bladder cancer treatement.
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| Overall design |
Examination of DNA methylation level of bladder cancer cells treated with lose-dose decitabine.
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| Contributor(s) |
Li Y, Li J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 23, 2018 |
| Last update date |
Apr 24, 2021 |
| Contact name |
Jiong Li |
| E-mail(s) |
[email protected]
|
| Phone |
3105617186
|
| Organization name |
UCLA
|
| Department |
School of Dentistry
|
| Lab |
Cunyu Wang
|
| Street address |
33-030 CHS
|
| City |
Los Angeles |
| State/province |
CA - California |
| ZIP/Postal code |
91406 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA451473 |
| SRA |
SRP142314 |
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