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| Status |
Public on Dec 01, 2021 |
| Title |
Characterization of a newly-identified myelinating oligodendroglial population expressing NDRG1 in the adult mouse central nervous system |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Oligodendrocytes are the main myelinating and remyelinating cells of the central nervous system. We identified N-myc downstream regulated gene family member 1 (NDRG1) as a specific mature marker of the oligodendroglial lineage, regulated by mTORC2 kinase activity. By generating a transgenic reporter murine line, we managed to precisely characterize the effect of cuprizone-induced treatment on myelinating cells. We also created a new and adult-specific whole genome transcriptomic profile of the oligodendroglial lineage, from proliferative OPC (using P60 PDGFRa::GFP sorted cells), to pre-myelinating OL (using P60 PLP::GFP sorted cells) and then mature OL (using P60 NDRG1-eGFP sorted cells). This new reporter murine line and its associated RNA-Sequencing dataset could help us better understand the specific biology of adult myelinating oligodendrocytes, compared to previously published developmental tools and databases.
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| Overall design |
mRNA profiles of FAC-sorted P60 PDGFRa::GFP, P60 PLP::GFP and P60 NDRG1-eGFP purified cell samples from mouse brains were generated by RNA-sequencing, in triplicate, using Illumina HiSeq 2500.
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| |
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| Contributor(s) |
Moyon S, Castro K, Patzig J, Gacias M, Magri L, Casaccia P |
| Citation(s) |
34687571 |
| |
| Submission date |
Apr 24, 2018 |
| Last update date |
Mar 02, 2022 |
| Contact name |
Sarah Moyon |
| E-mail(s) |
[email protected]
|
| Organization name |
CUNY ASRC
|
| Department |
Neuroscience
|
| Lab |
Casaccia
|
| Street address |
85 St Nicholas Terrace
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10031 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (9)
|
| GSM3110139 |
P60 brain oligodendrocyte progenitor cells_RNASeq_n1 |
| GSM3110140 |
P60 brain oligodendrocyte progenitor cells_RNASeq_n2 |
| GSM3110141 |
P60 brain oligodendrocyte progenitor cells_RNASeq_n3 |
| GSM3110142 |
P60 brain pre-myelinating oligodendrocytes_RNASeq_n1 |
| GSM3110143 |
P60 brain pre-myelinating oligodendrocytes_RNASeq_n2 |
| GSM3110144 |
P60 brain pre-myelinating oligodendrocytes_RNASeq_n3 |
| GSM3110145 |
P60 brain myelinating oligodendrocytes_RNASeq_n1 |
| GSM3110146 |
P60 brain myelinating oligodendrocytes_RNASeq_n2 |
| GSM3110147 |
P60 brain myelinating oligodendrocytes_RNASeq_n3 |
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| Relations |
| BioProject |
PRJNA453399 |
| SRA |
SRP142537 |
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