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| Status |
Public on Dec 31, 2018 |
| Title |
LRP5 and LRP6 are required for maintaining self-renewal and differentiation of hematopoietic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The canonical Wnt signaling pathway has been demonstrated as a critical role in the self-renewal, proliferation and differentiation of Hematopoietic Stem Cells (HSCs), but the functions are indeterminacy in adult HSCs since the different experimental systems using gain- or loss- functions mice models. Low-density lipoprotein receptor-related proteins 5 and 6 (LRP5 and LRP6) are important co-receptors in the canonical Wnt/β-catenin pathway. In this study, the knockout mice models were established for adult HSC investagation via conditional ablation of LRP5 and LRP6 in adult hematopoiesis by Vav-Cre Loxp system. We found the HSC numbers were diminished due to the decreased proliferation and cell cycle. To investigate the molecular mechanisms, RNA-seq was performed using HSC cells isolated from WT and dKO mice.
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| |
|
| Overall design |
HSC (CD34-Flt3- Lin-ScaI+C-Kithigh cells) purified by FACS sorting
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| |
|
| Contributor(s) |
Yu S, Liu J |
| Citation(s) |
30668923 |
| |
| Submission date |
Nov 16, 2018 |
| Last update date |
Apr 01, 2019 |
| Contact name |
Shuyang Yu |
| E-mail(s) |
[email protected]
|
| Organization name |
China Agricultural University
|
| Department |
College of Biological Sciences
|
| Street address |
2 Yuanmingyuan West Road
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
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| Platforms (1) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA505821 |
| SRA |
SRP169107 |
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