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| Status |
Public on Oct 15, 2019 |
| Title |
Transcriptional redirection of activated SKN-1/NRF2 abates the negative metabolic outcomes of a perceived pathogen infection |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Optimal health requires perpetual transcriptional fidelity of gene expression. SKN-1/NRF2 is a cytoprotective transcription factor in C. elegans that regulates the expression of cellular defenses during stress, including: nutrient deprivation, redox imbalance, and xenobiotic and pathogen exposure. Constitutive activation of SKN-1 results in pleiotropic outcomes, including shortened lifespan and protective redistribution of somatic fat to the germline. We measured lipid distribution between the soma and germ tissues after manipulation of SKN-1 activity. Modulating the epigenetic landscape refines SKN-1 activity away from innate immunity targets and alleviates negative metabolic outcomes. Similarly, paraquat exposure redirects SKN-1 activity toward oxidative stress responses and away from pathogen response genes, which restores lipid distribution across tissues. Lastly, activating p38 MAPK signaling is sufficient to drive SKN-1-dependent loss of somatic fat. These data reveal a coordination of organismal metabolic homeostasis with pathogen responses and identifies mechanisms for counteracting the pleiotropic consequences of aberrant transcriptional activity.
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| Overall design |
Our study aim to look at transcriptional signatures that changes in Day 3 adults, when loss of fat phenotype occurs in constitutively active SKN-1 worms compared to wild type. We are also interested in transcriptional changes when that loss of fat phenotype is suppressed by paraquat exposure or chromatin restriction.
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| Contributor(s) |
Nhan J, Curran S |
| Citation(s) |
31611372 |
| |
| Submission date |
Dec 10, 2018 |
| Last update date |
Nov 12, 2019 |
| Contact name |
Sean Curran |
| E-mail(s) |
[email protected]
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| Organization name |
University of Southern California
|
| Department |
Davis School of Gerontology
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| Lab |
Curran
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| Street address |
3715 McClintock Ave
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA509132 |
| SRA |
SRP173212 |
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