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| Status |
Public on Dec 19, 2019 |
| Title |
Transcriptional profiling of nutrient-balanced early trilineage differentiations derived from H9 hPSCs. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here, we show unexpected and crucial metabolic nodes through TCA flux and glutaminolysis that modulate germ layer specification. Previous reports have shown that sustained glycolytic flux though MYC regulation is required for ectoderm specification. Our data support the finding that the germ layers have distinct metabolic profiles, but through investigation into the role of OXPHOS in germ layer specification.
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| Overall design |
Bulk RNA-Sequencing of H9-derived hPSCs differentiated to early trilineage (mesoderm, endoderm, and ectoderm) using a nutrient-balanced media formulation. Samples represent n = 5-7 of each lineage (hPSC, ME, EN, and EC).
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| |
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| Contributor(s) |
Ahsan FM, Lu V, Teitell MA |
| Citation(s) |
31189991 |
| |
| Submission date |
Feb 27, 2019 |
| Last update date |
Dec 21, 2019 |
| Contact name |
Fasih Mubtasim Ahsan |
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Genomic Medicine
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| Lab |
Soukas Lab
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| Street address |
185 Cambridge Street, CPZN 6500
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA524638 |
| SRA |
SRP187040 |
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