|
| Status |
Public on Feb 26, 2020 |
| Title |
RNA-seq on miRNA induced mouse keratinocytes. miR-200b cluster and miR-205. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Vehicle versus Doxycycline miRNA induction.
|
| |
|
| Overall design |
miR-200 cells are K14rtTA/pTRE2-miR-200b cluster
miR-205 cells are K14rtTA/pTRE2-miR-205
For miR-200 induction, samples were sequenced on a Miseq and a Hiseq machine. Counts were then combined at the step of Htseq counts. This gave the most consistent results.
miR-200 experiments were done in duplicate and miR-205 were done in triplicates
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| |
|
| Contributor(s) |
Bjerke G, Yi R |
| Citation(s) |
31900330, 37216502 |
| |
| Submission date |
May 14, 2019 |
| Last update date |
Jun 07, 2023 |
| Contact name |
Glen Bjerke |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Colorado
|
| Department |
MCDB
|
| Lab |
Yi Lab
|
| Street address |
347 UCB
|
| City |
Boulder |
| State/province |
CO |
| ZIP/Postal code |
80301 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA542900 |
| SRA |
SRP198396 |
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