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| Status |
Public on Jul 29, 2019 |
| Title |
Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Translation is a basic cellular process and its capacity is adapted to cell function. In particular, secretory cells achieve high protein synthesis levels without triggering the protein stress response. It is unknown how and when translation capacity is increased during differentiation. Here, we show that the transcription factor Creb3l2 is a scaling factor for translation capacity in pituitary secretory cells and that it directly binds ~75% of regulatory and effector genes for translation. In parallel with this cell-autonomous mechanism, implementation of the physiological UPR pathway prevents triggering the protein stress response. The pituitary differentiation factor Tpit activates Creb3l2 expression, the Creb3l2-dependent regulatory network as well as the physiological UPR pathway. Thus, Creb3l2 implements high basal translation levels through direct targeting of translation effector genes acting downstream of signaling pathways that otherwise regulate protein synthesis. Expression of Creb3l2 may be a useful means to enhance production of therapeutic proteins.
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| |
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| Overall design |
ChIPseq in normal AtT-20 and Creb3l2 gain-of-function Tg corticotrope AtT-20 cells
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| |
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| Contributor(s) |
Khetchoumian K, Drouin J |
| Citation(s) |
31481663 |
| |
| Submission date |
Jun 06, 2019 |
| Last update date |
Sep 11, 2019 |
| Contact name |
Amandine Bemmo |
| E-mail(s) |
[email protected]
|
| Organization name |
IRCM
|
| Lab |
Dr. Drouin
|
| Street address |
110 Avenue des Pins
|
| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H2W 1R7 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (2) |
| GSM3856429 |
FLAG ChIPseq in AtT-20 cells expressing 3x flag-Creb3l2 (cleaved form) |
| GSM3856430 |
XBP1 ChIPseq in AtT-20 cells |
|
| This SubSeries is part of SuperSeries: |
| GSE132324 |
Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells |
|
| Relations |
| BioProject |
PRJNA547601 |
| SRA |
SRP200670 |
