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| Status |
Public on Mar 01, 2021 |
| Title |
Role of Transforming Growth Factor-β1 in Recessive Dystrophic Epidermolysis Bullosa Squamous Cell Carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this RNA-seq experiment was to determine how gene expression in RDEBSCC cell lines was altered relative to normal culture conditions when the culture media was supplemented with TGFb1 or TGFb1 and a TGFb1 inhibitor (SB431542).
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| Overall design |
Three cell lines derived from squamous cell carcinoma tumors in patients with recessive dystrophic epidermolysis bullosa were used (2, 4, and 70). RNA-seq was performed for three experimental conditions for each cell line. The three conditions were (1) media only, (2) media+TGFb1, and (3) media+TGFb1+SB431542 (TGFb1 inhibitor). Each experimental condition was replicated three times for each cell line, except condition 2 and 3 for cell line 2, where the samples were lost, resulting in a total of 25 paired-end samples.
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| Contributor(s) |
Twaroski K, Chen W, Pickett-Leonard M, Tolar J |
| Citation(s) |
33595864 |
| |
| Submission date |
Jul 17, 2019 |
| Last update date |
Jun 01, 2021 |
| Contact name |
Jakub Tolar |
| E-mail(s) |
[email protected]
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| Organization name |
University of Minnesota - Twin Cities
|
| Department |
Pediatrics
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| Lab |
Stem Cell Institute
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| Street address |
2001 6th St SE
|
| City |
Minneapolis |
| State/province |
Minnesota |
| ZIP/Postal code |
55455 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA555069 |
| SRA |
SRP215227 |
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