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| Status |
Public on Dec 25, 2022 |
| Title |
Transcriptome analysis of MCF-7 breast cancer cells in which VASP gene was knocked down |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Previous studies have shown that the main function of VASP is to regulate the cytoskeleton and play an important role in promoting tumor cell metastasis. In this study, we first reveal that VASP is located in the nucleus of breast cancer cells and elucidate a Wnt/β-catenin/VASP positive feedback loop. We identify that VASP is a target gene of Wnt/β-catenin signaling pathway, and activation of Wnt/β-catenin signaling pathway can significantly upregulate VASP protein expression, while upregulated VASP protein can in turn promote translocation of β-catenin and DVL3 proteins into the nucleus. In the nucleus, VASP, DVL3, β-catenin and TCF4 can form VASP/DVL3/β-catenin/TCF4 protein complex, activating Wnt/β-catenin signaling pathway, and promoting the expression of target genes VASP, c-myc and cyclin D1. Thus, our study reveals that there is a Wnt/β-catenin/VASP malignant positive feedback loop in breast cancer, which promotes the proliferation and migration of breast cancer cells, and breaking this positive feedback loop may provide new strategy for breast cancer treatment.
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| Overall design |
Examination of the effect of VASP knockdown on the human breast cancer cells MCF-7
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| Contributor(s) |
Li K, Wei L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 27, 2019 |
| Last update date |
Dec 25, 2022 |
| Contact name |
Kai Li |
| E-mail(s) |
[email protected]
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| Phone |
+8613297061851
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| Organization name |
Wuhan University
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| Street address |
185 Donghu Road, Wuchang District
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| City |
Wuhan |
| ZIP/Postal code |
430071 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE142703 |
Transcriptome analysis of breast cancer cells in which VASP gene was knocked down |
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| Relations |
| BioProject |
PRJNA562507 |
| SRA |
SRP219748 |
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