|
| Status |
Public on Feb 08, 2020 |
| Title |
Voltage-gated sodium channel (VGSC) beta1 subunit-intracellular domain (Beta1-ICD) overexpression in Chinese hamster lung cells |
| Organism |
Cricetulus griseus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Overexpression of hypothesized transcriptional regulator in a cell line to compare gene expression changes to in vivo knockout of upstream signaling pathway component. All samples are from Chinese hamster lung cells.
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| |
|
| Overall design |
RNA-Seq, 2 groups with 4 samples each. Four samples have stable overexpression of human VGSC beta1 subunit-intracellular domain (Beta1-ICD) tagged with V5 followed by a 2A peptide and eGFP (WTICD) and four controls have overexpression of eGFP alone (eGFP).
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| |
|
| Contributor(s) |
Isom L, Bouza A, Tagett R, King D |
| Citation(s) |
33411695 |
| |
| Submission date |
Sep 05, 2019 |
| Last update date |
Mar 16, 2021 |
| Contact name |
Bioinformatics Core |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan
|
| Street address |
BRCF Bioinformatics Core, 2800 Plymouth Rd
|
| City |
Ann Arbor |
| State/province |
Mi |
| ZIP/Postal code |
48109-2800 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL27425 |
Illumina NovaSeq 6000 (Cricetulus griseus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA564074 |
| SRA |
SRP220465 |
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