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Series GSE136987 Query DataSets for GSE136987
Status Public on Aug 26, 2022
Title Multiplex indexing approach for the detection of DNase I hypersensitive sites in single cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We describe a novel indexing protocol that employs the activities of terminal DNA transferase and T4 DNA ligase to add unique cell barcodes to DNase-digested DNA ends. This protocol allows profiling of genome-wide DHSs in over 10,000 single-cells in an individual experiment.
 
Overall design Profiling genome-wide DHSs with terminal DNA transferase and T4 DNA ligase
 
Contributor(s) Gao W, Ku WL, Perrie J, Hu G, Zhu J, Ni B, Zhao K
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Submission date Sep 06, 2019
Last update date Aug 26, 2022
Contact name Keji Zhao
E-mail(s) [email protected]
Phone 301-496-2098
Organization name NHLBI
Department Systems Biology Center
Lab Laboratory of Epigenome Biology
Street address 10 Center Dr
City Bethesda
State/province MD
ZIP/Postal code 20814
Country USA
 
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (480)
GSM4064466 human WBC, GB4030001 (iDNase-seq)
GSM4064467 human WBC, GB4030002 (iDNase-seq)
GSM4064468 human WBC, GB4030003 (iDNase-seq)
Relations
BioProject PRJNA564226
SRA SRP220632

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE136987_barcode_480.txt.gz 1.6 Kb (ftp)(http) TXT
GSE136987_iDNase_4489_cells_218042_peaks_counts_at_WBC.mtx.gz 27.8 Mb (ftp)(http) MTX
GSE136987_peaks_218042_WBC.txt.gz 1.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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