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| Status |
Public on Apr 18, 2020 |
| Title |
Vascular smooth muscle selective deletion of Srf |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Previous efforts to conditionally delete the Srf transcription factor in smooth muscle lineages were met with early demise of the mouse due to a gastrointestinal (GI) obstruction phenotype. We have developed a new, more VSMC selective Cre recombinase mouse (Itga8-CreERT2), that circumvents the GI phenotype thus affording the opportunity to evaluate, in an unambiguous manner, VSMC phenotypes where Srf is reduced. The mice live for at least 6 months post-tamoxifen and here we report the first RNA-seq experiments in mouse aortic SMC where Srf is reduced.
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| Overall design |
Mice homozygous or heterozygous for a floxed Srf allele and hemizygous for the Itga8-CreERT2 driver were treated with 5 daily doses of 33mg/kg Tamoxifen (i.p.). Aortae from the experimental homozygous cKO (n=3) or the control heterozygous cKO (n=3) were harvested 10 days following the last tamoxifen injection. Only 2 homozygous cKO replicates are reported here due to an outlier effect (or error in sampling) in one of the experimental samples. Thus, the data herein reflect only 2 homoygous cKOs and 3 heterozygous cKO controls.
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| Contributor(s) |
Lyu Q, Zhang W, Slivano OJ, Christie CK, Long X, Gan L, Miano JM |
| Citation missing |
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| |
| Submission date |
Oct 14, 2019 |
| Last update date |
Apr 18, 2020 |
| Contact name |
Joseph M Miano |
| E-mail(s) |
[email protected]
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| Organization name |
University of Rochester
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| Street address |
601 Elmwood Avenue
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| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA577451 |
| SRA |
SRP225543 |
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