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| Status |
Public on May 04, 2020 |
| Title |
circRNA expression in METTL3 and YTHDC1 KNOCK-DOWN conditions |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Through an siRNA based approach, we investigated the effect of the knock-down of METTL3 and YTHDC1 on circRNAs expression in HeLa cells
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| Overall design |
Total RNA from HeLa cells treated either with siRNA against METTL3 (two biological replicates) and control siRNA (one biological replicate), together with total RNA from HeLa cells treated with a siRNA against YTHDC1 (two biological replicates) and control siRNA (two biological replicates), was analyzed using Illumina Novaseq6000.
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| Contributor(s) |
Di Timoteo G, Dattilo D, Guarnacci M, Colantoni A, Centrón-Broco A, Rossi F, Morlando M, Bozzoni I |
| Citation(s) |
32402287 |
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| Submission date |
Oct 18, 2019 |
| Last update date |
Aug 04, 2020 |
| Contact name |
Gaia Di Timoteo |
| Organization name |
University of Rome "La Sapienza"
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| Street address |
piazza Aldo Moro 5
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| City |
Rome |
| State/province |
RM |
| ZIP/Postal code |
00185 |
| Country |
Italy |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA578279 |
| SRA |
SRP226173 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE139076_RAW.tar |
2.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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