|
| Status |
Public on Feb 01, 2022 |
| Title |
Identification of exosomal miRNAs specifically derived from intrafollicular cells involved in polycystic ovary syndrome |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Exosomes have recently been shown to play a key role in cell-to-cell communication through delivery of various functional content, including microRNAs (miRNAs). We investigated the potential roles of exosomal miRNA derived intrafollicular cells in polycystic ovary syndrome (PCOS). Using microarray profiling, a total of 492 miRNAs and 220 miRNAs were found in follicular fluid-derived exosomes and serum-derived exosomes, respectively, in PCOS and non-PCOS females. By excluding miRNAs existing in serum-derived exosomes, we found 179 miRNAs which were specifically expressed in follicular fluid-derived exosomes both in PCOS and non-PCOS females.
Using microarray profiling, a total of 492 miRNAs and 220 miRNAs were found in follicular fluid-derived exosomes and serum-derived exosomes, respectively, in PCOS and non-PCOS females. By excluding miRNAs existing in serum-derived exosomes, we found 179 miRNAs which were specifically expressed in follicular fluid-derived exosomes both in PCOS and non-PCOS females.
|
| |
|
| Overall design |
Exosomes were isolated from pooled follicular fluid of PCOS and non-PCOS females, respectively. Serum-derived exosomes were isolated from pooled serum of PCOS and non-PCOS females, respectively. miRNA profiles were generated using microarray. Comparison of miRNAs expression in fluid-derived exosomes and serum-derived exosomes were preformed to identify the exosomal miRNAs specifically derived from intrafollicular cells
|
| |
|
| Contributor(s) |
Cui C, Wang J, Zhang C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 14, 2020 |
| Last update date |
Feb 02, 2022 |
| Contact name |
Chenchen Cui |
| E-mail(s) |
[email protected]
|
| Organization name |
Henan Provincial People’s Hospital
|
| Department |
Department of Reproductive Medicine Center
|
| Street address |
Weiwu Road #7
|
| City |
Zhengzhou |
| State/province |
Henan |
| ZIP/Postal code |
450003 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL21576 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (Probe Name version) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA606756 |
Less...
More...