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| Status |
Public on Feb 18, 2023 |
| Title |
Nucleotide variation in histone H2BL promotes cell proliferation by upregulating c-Myc |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We found a histone H2B mutation, H2BL-T11C (leading to L3P amino acid variation) from tissue samples. Cells overexpressing H2BL-L3P showed stronger proliferation, colony formation and tumorigenic abilities. We further demonstrated that overexpressing H2BL-L3P made the cell cycle distribution changed via upregulation of c-Myc expression level. Furthermore, cell cycle studies based on EdU staining revealed the importance of c-Myc in promoting cell cycle progression through the G1/S checkpoint. As expected, treatment with 10058-F4, an inhibitor of the c-Myc-MAX interaction, alleviated the cell proliferation and cell cycle distribution phenotypes in the H2BL-L3P group and partially inhibited tumour growth. Overall, the amino acid mutation L3P, resulting from a SNP at a histone H2B locus, is associated with tumorigenesis and tumour progression. Additionally, the mechanisms and inhibitors involved in this study may provide a theoretical basis for targeted treatment of cancers with the same mutation.
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| Overall design |
Three independent replacations from each group of samples of SMMC-7721 cell lines (H2BG,H2BL and H2BL-L3P) with stable expression were collected and used for high-throughput RNA sequencing.
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| Contributor(s) |
Sun J, Zhang L |
| Citation missing |
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| Submission date |
Feb 18, 2020 |
| Last update date |
Feb 18, 2023 |
| Contact name |
Dade Song |
| E-mail(s) |
[email protected]
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| Phone |
17521689979
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| Organization name |
Advanced Institute of Qingdao,Tongji University
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| Street address |
No.1239,Siping Road
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| City |
Shanghai |
| ZIP/Postal code |
200000 |
| Country |
China |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA607245 |
| SRA |
SRP249806 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145448_readscount.summary.txt.gz |
378.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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