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| Status |
Public on Oct 10, 2021 |
| Title |
Single-cell RNA-seq analysis of prostate cancer cells [II] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Androgen receptor (AR) plays key roles both in development of normal prostate gland and at all stages of prostate cancer. Due to the diversity of cell response to androgen stimulation, it’s necessary to investigate AR regulatory network at single cell level to fully understand how AR regulates transcription in prostate cancer cells. Here, we performed normal growing and time-series 5α-dihydrotestosterone (DHT) stimulated single cell RNA-seq profiling and transcriptome analysis on prostate cancer cell lines. We sequenced 186 single cell transcriptomes in total and found gene expression heterogeneity among each cell. Finally, novel biological processes have been discovered combined with clinical data. This study provides a comprehensive profile to dissect hormone regulation in prostate cancer at single cell resolution.
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| |
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| Overall design |
Single-cell RNA and bulk sequencing of normal growing VCaP and LNCaP, time course LNCaP cells stimulated by DHT.
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| |
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| Contributor(s) |
Hu L, Chen X, Cheung E |
| Citation(s) |
34611310 |
| |
| Submission date |
Feb 24, 2020 |
| Last update date |
Oct 10, 2021 |
| Contact name |
Xin Chen |
| Organization name |
Guangdong University of Technology
|
| Street address |
No. 100 Waihuan Xi Road, GuangzhouHigher Education Mega Center, Panyu District
|
| City |
Guangzhou |
| ZIP/Postal code |
510006 |
| Country |
China |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (192)
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| This SubSeries is part of SuperSeries: |
| GSE145845 |
Single-cell RNA-seq analysis of prostate cancer cells |
|
| Relations |
| BioProject |
PRJNA608497 |
| SRA |
SRP250577 |
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