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Series GSE146057 Query DataSets for GSE146057
Status Public on Jan 13, 2021
Title Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos (RNA-seq)
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary Inheritance and clearance of maternal mRNAs are two of the most critical events required for animal early embryonic development. However, the mechanisms regulating this process are still largely unknown. Here, we show that together with maternal mRNAs, C. elegans embryos inherit a complementary pool of small non-coding RNAs that facilitate the cleavage and removal of hundreds of maternal mRNAs. These antisense small RNAs are loaded into the maternal catalytically-active Argonaute CSR-1 and cleave complementary mRNAs no longer engaged in translation in somatic blastomeres. Induced depletion of CSR-1 specifically during embryonic development leads to embryonic lethality in a slicer-dependent manner and impairs the degradation of CSR-1 embryonic mRNA targets. Given the conservation of Argonaute catalytic activity, we propose that a similar mechanism operates to clear maternal mRNAs during the maternal-to-zygotic transition across species.
 
Overall design 33 samples were analyzed by RNA-seq, including replicates (sample name contains 'rep').
 
Contributor(s) Quarato P, Singh M, Cornes E, Li B, Didier C, Bourdon L, Mueller F, Cecere G
Citation(s) 33664268
Submission date Feb 27, 2020
Last update date Apr 06, 2021
Contact name Germano Cecere
E-mail(s) [email protected]
Phone 0033140613225
Organization name Institut Pasteur
Department Development and stem cell biology
Lab Mechanisms of Epigenetic Inheritance
Street address Institut Pasteur, 28 Rue Du Docteur Roux, Batiment Monod, 4eme Etage
City Paris
ZIP/Postal code 75724
Country France
 
Platforms (1)
GPL19757 Illumina NextSeq 500 (Caenorhabditis elegans)
Samples (33)
GSM4363820 CSR-1_degron_embryos_rep1_RNA-seq
GSM4363821 CSR-1_degron_embryos_rep2_RNA-seq
GSM4363822 CSR-1_degron_embryos_control_rep1_RNA-seq
This SubSeries is part of SuperSeries:
GSE146062 Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos
Relations
BioProject PRJNA609219
SRA SRP251098

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Supplementary file Size Download File type/resource
GSE146057_RAW.tar 644.9 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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