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| Status |
Public on Sep 08, 2020 |
| Title |
Genome-wide mapping of the heterochromatin mark H3K9me2 in loss-of-function mutant worms |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Histone modification marks such as H3K4me1 and H3K27ac have been used to map putative enhancers, but these marks represent only a fraction of the full repertoire of histone modifications decorating these regulatory regions. In mammals, methylation of histone H3 on lysine 79 (H3K79me), deposited by the methyltransferase DOT1L, is present at enhancers. In C. elegans, putative enhancers have been predicted based on chromatin modifications and regions of chromatin accessibility. Our own overlap analysis has revealed that both DOT-1.1, the C. elegans DOT1L homologue, and its major interacting partner, the chromatin-binding factor ZFP-1 (AF10 in mammals), are enriched at both distal and intragenic enhancers. Previous work has shown that Dot1 proteins prevent encroachment of heterochromatin to actively transcribed regions in yeast and in MLL-rearranged leukemias. Here we find that, in C. elegans, loss of DOT-1.1 is accompanied by ectopic H3K9me2, a hallmark of heterochromatin, at distal and intragenic enhancers. This observation suggests a new role for DOT1L in the control of heterochromatin formation at enhancers, which may partake on the control of developmentally regulated genes.
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| Overall design |
Profiling of H3K9me2 in wild-type (WT) and mutant (zfp-1, ced-3, dot-1.1; ced-3, rde-1; dot-1.1, and rde-4; dot-1.1) third larval (L3) stage Caenorhabditis elegans
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| Contributor(s) |
Esse R, Grishok A |
| Citation(s) |
32781660 |
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| Submission date |
May 12, 2020 |
| Last update date |
Sep 08, 2020 |
| Contact name |
Ruben Esse |
| E-mail(s) |
[email protected]
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| Organization name |
King’s College London
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| Department |
Department of Medical and Molecular Genetics
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| Street address |
Guy's Hospital, Great Maze Pond
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| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19757 |
Illumina NextSeq 500 (Caenorhabditis elegans) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA631996 |
| SRA |
SRP261312 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE150407_RAW.tar |
353.6 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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