|
| Status |
Public on Jul 28, 2021 |
| Title |
Yeast three hybrid experiment |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
Expanding the binding specificity for RNA recognition by a PUF domain
|
| |
|
| Overall design |
We used next generation sequencing to score the binding activity of each variant for its ability to bind to an RNA sequence containing each possible RNA base at the cognate position, which identified many variants with highly repeat-specific interactions.
|
| |
|
| Contributor(s) |
Zhou W, Melamed D, Wickens M, Fields S |
| Citation(s) |
34429425 |
| |
| Submission date |
Jun 15, 2020 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Wei Zhou |
| Organization name |
University of Washington
|
| Department |
genome sciences
|
| Lab |
Stanley Fields
|
| Street address |
William H. Foege Hall, 3720 15th Ave NE
|
| City |
SEATTLE |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA639454 |
| SRA |
SRP267315 |
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