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| Status |
Public on Mar 24, 2010 |
| Title |
Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome 2 |
| Organism |
Glycine max |
| Experiment type |
Expression profiling by array
|
| Summary |
Atrazine and bentazon are both photosystem II inhibiting herbicides that interfere with photosynthetic electron transport provoking oxidative stress. While atrazine is lethal to soybean, bentazon does not kill soybeans because of the capability of soybeans to metabolize the herbicide. Gene expression profiling was conducted using cDNA microarrays to understand the responses of soybeans to PSII interruption and concomitant oxidative stress caused by atrazine and bentazon by monitoring expression at 1, 2, 4, and 8 h after treatment (HAT). The microarray study revealed that 6,646 genes were differentially expressed with high statistical significance over the experiment with 88% of them sharing similar expression pattern between the atrazine and bentazon treatments. Many genes related to xenobiotic detoxification and antioxidation such as cytochrome P450s, glutathione-S-transferases, superoxide dismutases, catalases and tocopheral cyclases were induced by the herbicides. The study also discovered plants treated with bentazon started to recover between 4 HAT and 8 HAT as reflected in the decreased amplitude of fold changes of most genes from 4 to 8 HAT. The 12% of the genes that were differentially expressed between atrazine and bentazon were largely related to cell recovery, such as genes related to ribosomal components.
Keywords = stress
Keywords = GST
Keywords = ROS
Keywords = D1 protein
Keywords = Fuzzy k-means
Keywords = plant
Keywords: plant xenobiotic interaction
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| Overall design |
loop design, 12 direct comparisons, 2 technical repeats including dye swaps, 3 biological repeats
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| |
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| Contributor(s) |
Zhu J, Patzoldt WL, Radwan O, Tranel PJ, Clough SJ |
| Citation |
Jin Zhu, William L. Patzoldt, Osman Radwan, Patrick J. Tranel, and Steven J. Clough. 2009. Effects of photosystem-II-interfering herbicides atrazine and bentazon on the soybean transcriptome. The Plant Genome. 2:191-205.
doi:10.3835/plantgenome2009.02.0010
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| |
| Submission date |
Mar 24, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
min li |
| Organization name |
University of Illinois at Urbana-Champaign
|
| Lab |
Dr. Steve Clough
|
| Street address |
Rm230 1101 W peabody Dr
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6009 |
Keck Glycine max 18kB cDNA array_prints 201-206 |
|
| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE15377 |
Effects of Photosystem II Interfering Herbicides Atrazine and Bentazon on the Soybean Transcriptome |
|
| Relations |
| BioProject |
PRJNA122943 |
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