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| Status |
Public on Jul 07, 2020 |
| Title |
RNA-seq for two forms of Gcr1, during exponential phase and diauxic shift |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Gcr1, the most important transcription factor for glycolytic genes in Saccharomyces cerevisiae, was recently revealed to have two isoforms, Gcr1(U) and Gcr1(S), produced from unspliced and spliced transcripts, respectively. We generated strains expressing only Gcr1(U) or Gcr1(S) form using the CRISPR/Cas9 system, and performed RNA-seq for the two strains. Since Gcr1 works as a glycolytic gene transcription factor, we extracted whole RNA at the exponential phase (6 hrs after incubation) and the diauxic shift (12 hrs after incubation).
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| Overall design |
The data contains the result of RNA-seq Gcr1 transcription factor of S. cerevisiae, which is known to have two forms in accordance with the intron processing (Gcr1(U) and Gcr1(S)). Duplicated RNA-seq sampling was conducted at two time points; during exponential phase (6 hr after incubation) and after diauxic shift (12 hr after incubation), for Gcr1(U) and Gcr1(S) each.
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| Contributor(s) |
Hahn J, Cha S, Hong CP |
| Citation missing |
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| BioProject |
PRJNA639179 |
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| Submission date |
Jul 06, 2020 |
| Last update date |
Jul 07, 2020 |
| Contact name |
seungwoo cha |
| E-mail(s) |
[email protected]
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| Phone |
+82-2-880-9297
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| Organization name |
Seoul National University
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| Department |
School of Chemical and Biological Engineering
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| Street address |
1 Gwanak-ro, Gwanak-gu
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| City |
seoul |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE153843 |
ChIP-seq and RNA-seq for two forms of Gcr1, during exponential phase and diauxic shift |
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| Relations |
| SRA |
SRP267142 |
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