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| Status |
Public on Jul 13, 2023 |
| Title |
Gene Expression Signature in Rat Pituitary MMQ Cells Treated With DRD2 agonist UNC9994 |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
DRD2 agonists are effective in treating pituitary tumors. To fathom the β-arresin-dependent mechanisms underlying DRD2-mediated pituitary tumor growth suppression,we applied RNA-seq analysis to rat pituitary MMQ cells treated with a DRD2 β-arresin-biased agoinst, UNC9994.MMQ cells were treated with UNC9994(15μM) or vehecle control for 12 h or 24 h and then subject to RNA-seq analysis.We found oxidative-stress-related genes such as Nqo1 and Hmox1 were upregulated by UNC9994, revealing oxidative stress may be the basis of UNC9994-induced tumor growth suppression. Our study represents the first detailed analysis of transcriptomes in rat pituitary MMQ cells treated with DRD2 β-arresin-biased agonist.The significance of altered expression of specific transcripts will enhance our understanding of DRD2 signaling in pituitary tumor cells.
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| Overall design |
MMQ cells were treated with UNC9994 (15μM) or vehicle control (DMSO) for 12h or 24h
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| Contributor(s) |
Tan Z |
| Citation missing |
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| |
| Submission date |
Jul 13, 2020 |
| Last update date |
Jul 13, 2023 |
| Contact name |
Zhoubin Tan |
| Organization name |
Tongji Hospital, Wuhan, China
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| Street address |
1095
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| City |
Wuhan |
| State/province |
Hubei province |
| ZIP/Postal code |
430000 |
| Country |
China |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA645928 |
| SRA |
SRP271651 |
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