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| Status |
Public on Dec 09, 2020 |
| Title |
Transcriptional effects of MAF- and MAFB-siRNAs on M-CSF-derived macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Analysis of the role of transcriptions factors MAF and MAFB on the phenotypic profles of human M-CSF-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) for 7 days in the presence of 10 ng/ml M-CSF to generate M-CSF-polarized macrophages (M-MØ). Macrophages were differentiated from peripheral blood monocytes from 3 healthy donors with M-CSF (M-MØ) to generate anti-inflammatory M-MØ. Macrophages were transfected with either Control siRNA or MAFB-specific siRNA or MAF-specific siRNA for 24h and global gene expression was analysed by RNA-Seq.
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| Overall design |
mRNA profiles of human M-CSF-derived macrophages treated with siRNA-MAF or siRNA-MAFB
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| |
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| Contributor(s) |
Dominguez-Soto A, Vega MA, Corbí AL |
| Citation(s) |
33312178, 36930354 |
|
https://doi.org/10.3389/fimmu.2020.603507
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| |
| Submission date |
Aug 05, 2020 |
| Last update date |
Jul 14, 2023 |
| Contact name |
Miguel A Vega |
| E-mail(s) |
[email protected]
|
| Organization name |
CIB (CSIC)
|
| Street address |
Ramiro de Maeztu 9
|
| City |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA655401 |
| SRA |
SRP276044 |
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