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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 25, 2021 |
| Title |
Elucidation of tumor-stromal heterogeneity and the ligand-receptor interactome by single cell transcriptomics in real-world pancreatic cancer biopsies |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Through single-cell RNA sequencing (scRNA-seq), we identify substantial transcriptomic evolution of PDOs propagated from the parental tumor, which may alter predicted drug sensitivity. In addition, we performed an integrative analyses of PDAC biopsies and provide an in-depth characterization of the heterogeneity within the tumor microenvironment, including cancer-associated fibroblast (CAF) subclasses, and predicts for a multitude of ligand-receptor interactions, revealing potential targets for immunotherapy approaches.
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| Overall design |
Single cell RNA sequencing analysis of 9 biopsies, 8 PBMCs.
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| Contributor(s) |
Lee JJ, Bernard V, Semaan A, Monberg ME, Huang J, Stephens BM, Lin D, Rajapakshe KI, Weston BR, Bhutani MS, Haymaker CL, Bernatchez C, Taniguchi CM, Maitra A, Guerrero PA |
| Citation(s) |
34426439 |
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| Submission date |
Aug 18, 2020 |
| Last update date |
May 08, 2023 |
| Contact name |
Anirban Maitra |
| E-mail(s) |
[email protected]
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| Organization name |
The University of Texas MD Anderson Cancer Center
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| Department |
Translational Molecular Pathology
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| Lab |
Maitra laboratory
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| Street address |
6565 MD Anderson Blvd
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA657844 |
| SRA |
SRP277925 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE156405_RAW.tar |
337.9 Mb |
(http)(custom) |
TAR (of TAR) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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