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| Status |
Public on Dec 06, 2021 |
| Title |
Transcriptional signature of BCG vaccination |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The century-old Mycobacterium bovis Bacillus Calmette-Guerin (BCG) remains the only licensed vaccine against tuberculosis (TB). Despite this, there is still a lot to learn about the immune response induced by BCG, both in terms of phenotype and specificity. Here, we investigated the BCG-specific gene expression changes induced in PBMCs and CD4 memory T cells by BCG in individuals pre- and 8m post vaccination. We also determined whether reactivity against a peptide pool defined in individuals with controlled latent TB infection (MTB300), and with peptides homologous to peptides found in BCG, was boosted following BCG vaccination.
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| Overall design |
RNAseq of PBMC and sorted memory CD4 T cells from cryopreserved PBMC of 7 individuals who received BCG vaccination. Samples were taken pre- and post vaccination.
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| Contributor(s) |
Singhania A, Dubelko P, Lindestam Arlehamn C |
| Citation(s) |
34902786 |
| |
| Submission date |
Aug 18, 2020 |
| Last update date |
Dec 22, 2021 |
| Contact name |
Paige Dubelko |
| E-mail(s) |
[email protected]
|
| Organization name |
La Jolla Institure for Immunology
|
| Lab |
Peters lab
|
| Street address |
9420 Athena Circle
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (78)
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| Relations |
| BioProject |
PRJNA657864 |
| SRA |
SRP277944 |
| SRA |
SRP349329 |
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