|
| Status |
Public on Apr 29, 2021 |
| Title |
CTCF HiChIP data in A673 Ewing sarcoma cell lines (siCT & siSA2) |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
STAG2, a member of cohesin, is one of the most recurrently mutated genes in human cancer. Here, we investigated STAG2 function in the context of Ewing sarcoma, an aggressive bone tumor driven by EWS-FLI1 oncogene chimeric transcription factor. A673 Ewing sarcoma cell line was trasfected with siCT or 2 different siRNA targetting STAG2 during 72h and CTCF HiChIP experiments were preformed for each conditions. Analyses of HiChIP data show that STAG2 knowck-down alters CTCF-anchored loop extrusion.
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| Overall design |
A673 Ewing sarcoma cell line was trasfected with siCT or 2 different siRNAs targeting STAG2 (siSA2#6 or siSA2#8) during 72h. CTCF HiChIP experiments were preformed for each conditions.
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| |
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| Contributor(s) |
Surdez D, Zaidi S, Grossetête S, Raynal V, Baulande S, Hill V, Delattre O |
| Citation(s) |
33930311 |
| |
| Submission date |
Aug 21, 2020 |
| Last update date |
Jul 29, 2021 |
| Contact name |
Olivier Delattre |
| Organization name |
Institut Curie
|
| Department |
U830
|
| Lab |
Olivier Delattre
|
| Street address |
26 rue d'ulm
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (16)
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| GSM4735769 |
A673_siCT-Dh1_72h_HiChIP_CTCF_Rep1_1_Ad D347C075 |
| GSM4735770 |
A673_siCT-Dh1_72h_HiChIP_CTCF_Rep1_2_Ad D347C076 |
| GSM4735771 |
A673_siCT-Dh1_72h_HiChIP_CTCF_Rep2_Ad D347C077 |
| GSM4735772 |
A673_siCT-Dh1_72h_HiChIP_CTCF_Rep1_1_Ad D362C75 |
| GSM4735773 |
A673_siCT-Dh1_72h_HiChIP_CTCF_Rep1_2_Ad D362C76 |
| GSM4735774 |
A673_siCT-Dh1_72h_HiChIP_CTCF_Rep2_Ad D362C77 |
| GSM4735775 |
A673_siSA2-Dh6_72h_HiChIP_CTCF_Rep1_1_Ad D347C078 |
| GSM4735776 |
A673_siSA2-Dh6_72h_HiChIP_CTCF_Rep1_2_Ad D347C079 |
| GSM4735777 |
A673_siSA2-Dh6_72h_HiChIP_CTCF_Rep2_Ad D347C080 |
| GSM4735778 |
A673_siSA2-Dh6_72h_HiChIP_CTCF_Rep1_1_Ad D362C78 |
| GSM4735779 |
A673_siSA2-Dh6_72h_HiChIP_CTCF_Rep1_2_Ad D362C79 |
| GSM4735780 |
A673_siSA2-Dh6_72h_HiChIP_CTCF_Rep2_Ad D362C80 |
| GSM4735781 |
A673_siSA2-Dh8_72h_HiChIP_CTCF_Rep1_Ad D347C081 |
| GSM4735782 |
A673_siSA2-Dh8_72h_HiChIP_CTCF_Rep2_Ad D347C082 |
| GSM4735783 |
A673_siSA2-Dh8_72h_HiChIP_CTCF_Rep1_Ad D362C81 |
| GSM4735784 |
A673_siSA2-Dh8_72h_HiChIP_CTCF_Rep2_Ad D362C82 |
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| This SubSeries is part of SuperSeries: |
| GSE133228 |
STAG2 promotes CTCF-anchored loop extrusion and cis-promoter and -enhancer interactions |
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| Relations |
| BioProject |
PRJNA658584 |
| SRA |
SRP278404 |
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