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| Status |
Public on Oct 22, 2020 |
| Title |
Increased IL-6 Trans-Signaling in the Airways After an Allergen Challenge |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The pro-inflammatory cytokine IL-6 is elevated in both blood and airways in asthma. IL-6 signaling is regulated by its receptor composed of two proteins, IL-6R and GP130, that are found in both membrane and soluble forms. The interaction of IL-6 with soluble IL-6R (sIL-6R) can trigger IL-6 trans-signaling in cells such as epithelial cells and fibroblasts, which express GP130, but do not produce sufficient IL-6R. We aimed to analyze IL-6 trans-signaling in the airways after an allergen challenge and to examine the potential source of sIL-6R and sGP130. In addition to protein analysis of bronchoalveolar lavages by ELISA and flow cytometry, we performed next-generation RNA sequencing (RNA-seq) to study the expression of the IL-6R subunits by eosinophils activated by IL-3 in vitro. RNA-seq and proteomic analyses confirmed that eosinophils do not produce GP130 but produce both IL-6R and ADAM10, a metalloproteinase that cleaves membrane IL-6R into sIL-6R.
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| Overall design |
Gene expression profiles of blood eosinophils from three subjects with allergy and mild asthma that were cultured in RPMI and 10% serum with or without IL-3 (2 ng/ml) for 4 h and 20 h.
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| Contributor(s) |
Esnault S, Bochkov Y |
| Citation(s) |
33471392 |
| |
| Submission date |
Oct 21, 2020 |
| Last update date |
Jan 21, 2021 |
| Contact name |
Yury A Bochkov |
| E-mail(s) |
[email protected]
|
| Phone |
608-263-8553
|
| Organization name |
University Of Wisconsin-Madison
|
| Department |
Pediatrics
|
| Street address |
600 Highland Ave, K4/945 CSC
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53792-9988 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA670482 |
| SRA |
SRP288204 |
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