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| Status |
Public on Oct 01, 2021 |
| Title |
Knockdown of lncRNA ELF3-AS1 in two gastric cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our data showed that ELF3-AS1 was a nuclear lncRNA, and its biofunction in cancers is largely unknown to date. To explore the function of lncRNA ELF3-AS1 in gastric cancer, loss-of-function study was performed in SGC7901 and AGS cell lines. RNA sequencing studies showed that the expression of almost all the histone coding genes were significantly increased after knocking down of ELF3-AS1 in SGC7901 and AGS cell lines.
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| Overall design |
To reveal the possible target genes of ELF3-AS1 in gastric cancer, we transfected ELF3-AS1-targeted siRNAs and corresponding negative control siRNAs in the SGC7901 and AGS cell lines. The potent ELF3-AS1 target genes were identified by deep sequencing, using BGIseq500.
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| Contributor(s) |
Qin S, Li D |
| Citation(s) |
36457025 |
| |
| Submission date |
Nov 11, 2020 |
| Last update date |
Dec 02, 2022 |
| Contact name |
Qin Shanshan |
| E-mail(s) |
[email protected]
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| Organization name |
Institute of Basic Medical Sciences, School of Basic Medical Sciences, Hubei University of Medicine
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| Street address |
Renmin Road 30, Maojian District
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| City |
Shiyan |
| ZIP/Postal code |
442000 |
| Country |
China |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA676142 |
| SRA |
SRP292212 |
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