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| Status |
Public on Dec 13, 2023 |
| Title |
Generation and Characterization of Stable Pig Pre-gastrulation Epiblast Stem Cell Lines [single-cell RNA-seq] |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Despite ongoing attempts since the 1990s, no stable embryonic stem cell line has been established in pigs. Here, guided by insights from large-scale single-cell RNA-sequencing profiling of pig embryos from embryonic day (E) 0 to E14, we developed an in vitro culture system for establishing and maintaining stable pluripotent stem cell lines from pig E10 pre-gastrulation epiblasts (pgEpiSCs). Enabled by chemical inhibition of WNT-related signaling in culture, the pgEpiSCs maintain their pluripotency and normal karyotypes after more than 200 passages. Strikingly, ultra-deep in situ Hi-C analysis revealed functional impacts of 3D-spatial associations on the transcriptional regulation of pluripotency marker genes in the pgEpiSCs. Practically, we confirmed that the pgEpiSCs can readily tolerate at least three rounds of successive gene editing and generated cloned gene-edited live piglets. Our findings deliver on the long-anticipated promise of large model animal stem cells and open new avenues for biological research, animal husbandry, and biomedicine.
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| Overall design |
We collected pre-implantation porcine embryos (E0-E14) and pluripotent stem cell lines from porcine E10 pre-gastrulation epiblasts(pgEpiSCs) conducted single cell transcriptome sequencing.
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| Contributor(s) |
Han J |
| Citation missing |
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| |
| Submission date |
Mar 02, 2021 |
| Last update date |
Dec 13, 2023 |
| Contact name |
Jianyong Han |
| E-mail(s) |
[email protected]
|
| Organization name |
China Agricultural University
|
| Department |
State Key Laboratory of Agrobiotechnology
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| Lab |
Han lab
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| Street address |
2rd, Yuanmingyuan West Road
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| City |
Beijing |
| ZIP/Postal code |
100083 |
| Country |
China |
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| Platforms (1) |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
| GSE168107 |
Generation and Characterization of Stable Pig Pre-gastrulation Epiblast Stem Cell Lines |
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| Relations |
| BioProject |
PRJNA706353 |
| SRA |
SRP309085 |
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