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| Status |
Public on Dec 31, 2021 |
| Title |
Small RNA sequencing Analysis of adipose in six month calf Chinese swamp buffalo |
| Organism |
Bubalus bubalis |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Background: Intramuscular fat (IMF) content is an important index for beef quality. However, the genetics of IMF deposition is complex and still largely unclear, especially in buffalo. To identify miRNAs with potential regulatory role in lipid accumulated in muscle, we performed small RNA sequencing and identified miRNAs expressed in the longissimus dorsi muscle and back fat of Chinese buffalo, which provided vital information for further identification of miRNAs with potential regulatory role in the lipid accumulated in muscle. Results: Three small RNA libraries were constructed. A total of 32762032 raw reads were obtained from adipose groups, respectively. After filtering the adaptor and low quality reads, 32054381 clean reads were retained. In total, 623 miRNAs were identified.
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| Overall design |
Three adipose samples from Chinese swamp buffalo were collected from Benniu abattoir, a local slaughterhouse of Xinayang, P.R. China.
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| Contributor(s) |
Wang S, Ma Y |
| Citation missing |
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| Submission date |
Mar 30, 2021 |
| Last update date |
Dec 31, 2021 |
| Contact name |
Yun Ma |
| E-mail(s) |
[email protected]
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| Organization name |
Xinyang Normal University
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| Street address |
Shihe
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| City |
Xinyang |
| ZIP/Postal code |
464100 |
| Country |
China |
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| Platforms (1) |
| GPL25494 |
Illumina HiSeq 2500 (Bubalus bubalis) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA718548 |
| SRA |
SRP312734 |
